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Problematik der Chromosomenstruktur von Mesostoma

Problems of the chromosome structure of Mesostoma

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Abstract

Metaphase chromosomes of Mesostoma ehrenbergi and M. lingua when swollen attain sizes many times their normal one and form ramifications if they are treated with nuclear isolation medium or destilled water. After spreading and pronase digestion it can be demonstrated that the ramifications and ribbon like structures are formed by chromosomal protein and that they are partly free of chromosomal fibers. The chromosomal fibers (DNA and rest of protein) with a diameter of about 150 Å are composed of two sub-units about 70 Å in diameter. Any one subunit can be reduced to a single DNA containing strand of 30 Å by pronase digestion. The double strandedness is confirmed by chromatid isolabeling in 3H-thymidine-autoradiographs.

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Authors and Affiliations

  1. Institut für Genetik der Ruhr-Universität Bochum, Bundesrepublik Deutschland

    H. -G. Keyl & F. Göltenboth

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  1. H. -G. Keyl
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  2. F. Göltenboth
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Mit Unterstützung durch die Deutsche Forschungsgemeinschaft.

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Keyl, H.G., Göltenboth, F. Problematik der Chromosomenstruktur von Mesostoma . Chromosoma 36, 292–304 (1972). https://doi.org/10.1007/BF00283248

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  • DOI: https://doi.org/10.1007/BF00283248

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