Summary
By mutagenizing an E. coli strain carrying an amber suppressor supD - (or su I +), we isolated a mutant whose amber suppressor activity was now temperature-sensitive. The mutant suppressor gene was named sup-126, which was found to be cotransduced with the his gene by phage P1vir at the frequency of ca. 20%. At 30° C it suppresses many amber mutations of E. coli, phage T4, and phage λ. At 42° C, however, it can suppress none of over 30 amber mutations tested so far. The sup-126 mutation is unambiguous and stable enough to be useful for making production of an amber protein temperature-sensitive.
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Nagata, T., Horiuchi, T. Isolation and characterization of a temperature-sensitive amber suppressor mutant of Escherichia coli K12. Molec. Gen. Genet. 123, 77–88 (1973). https://doi.org/10.1007/BF00282991
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DOI: https://doi.org/10.1007/BF00282991