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Nucleotide sequence of a new class A β-lactamase gene from the chromosome of Yersinia enterocolitica: Implications for the evolution of class A β-lactamases

Molecular and General Genetics MGG volume 228, pages 215–220 (1991)Cite this article

Summary

The nucleotide sequence has been determined of a 1400 by fragment from the chromosome of Yersinia enterocolitica containing the gene for β-lactamase I. An ORF of 882 by was identified, which could code for a polypeptide of 294 amino acids, closely related to other β-lactamases of molecular class A. Amino acids 1–30 could constitute a signal peptide. The mature protein would be 264 amino acids long with a calculated pI of 6.2. Alignment of the amino acid sequence of the class A β-lactamases suggested the existence of two subgroups in the same class, and this is discussed in the context of the evolution of the enzymes.

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Affiliations

  1. Departamento de Biología Molecular F. de Medicina, Universidad de Cantabria, Cardenal Herrera Oria s/n, 39011, Santander, Spain

    Asunción Seoane & Juan M. García Lobo

Authors
  1. Asunción Seoane
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  2. Juan M. García Lobo
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Additional information

This sequence will appear in the EMBL Data Library under the accession number X57074

Communicated by W. Goebel

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Seoane, A., García Lobo, J.M. Nucleotide sequence of a new class A β-lactamase gene from the chromosome of Yersinia enterocolitica: Implications for the evolution of class A β-lactamases. Molec. Gen. Genet. 228, 215–220 (1991). https://doi.org/10.1007/BF00282468

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  • DOI: https://doi.org/10.1007/BF00282468

Key words

  • Yersinia enterocolitica
  • Class A β-lactamases