Summary
The dedB gene of Escherichia coli has sequence similarity to the zfpA gene of the chloroplast chromosome. The functions of dedB and zfpA are unknown. We constructed derivatives of temperature-sensitive polA strains into whose chromosomes a plasmid containing the disrupted dedB gene was integrated by homologous recombination. These strains contained normal and disrupted dedB genes in their chromosomes. We then selected plasmid-segregated strains and found no cells containing the disrupted dedB gene, indicating that disruption of the dedB gene was lethal in polA strains of E. coli.
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Nagano, Y., Matsuno, R. & Sasaki, Y. An essential gene of Escherichia coli that has sequence similarity to a chloroplast gene of unknown function. Molec. Gen. Genet. 228, 62–64 (1991). https://doi.org/10.1007/BF00282448
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DOI: https://doi.org/10.1007/BF00282448