Abstract
The positions of the endonucleolytic cleavages promoted by the integrase protein (Int) of coliphage HK022 within its attB site were determined. The protein catalyses a staggered cut, which defines an overlap sequence of 7 by within the core site. The overlap region is at the center of symmetry of a palindromic sequence which appears in all four putative att core binding sites for Int. We confirm that the order of strand exchange is similar to that in phage λ.
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Communicated by W Goebel
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Kolot, M., Yagil, E. Position and direction of strand exchange in bacteriophage HK022 integration. Molec. Gen. Genet. 245, 623–627 (1994). https://doi.org/10.1007/BF00282225
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DOI: https://doi.org/10.1007/BF00282225