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Immunoprecipitation of a male-specific polypeptide after in vitro translation of testicular poly(A)+ RNA

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Summary

Poly(A)+ RNAs from male and female gonads of 20-day-old rats were translated in vitro using rabbit reticulocyte lysates. The resulting polypeptides were incubated with specific anti-male (anti-H-Y) antisera raisedin female rats by intrasplenic immunization with syngeneic male skin. Using a second antibody, a male-specific polypeptide of molecular weight approximately 18,000 was immunoprecipitated. This male-specific polypeptide was not cotranslationally modified in vitro and appeared to be identical to serological H-Y antigen.

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Authors and Affiliations

  1. Abteilung für Gynäkologie und Geburtshilfe der Rheinisch-Westfälischen Technischen Hochschule, Pauwelsstrasse, D-5100, Aachen, Federal Republic of Germany

    Ulrich Lattermann

  2. Institut für Humangenetik und Anthropologie der Universität, Albertstrasse 11, D-7800, Freiburg i. Br., Federal Republic of Germany

    Ulrich Lattermann

  3. Genetics Division, The Children's Hospital Medical Center, 02115, Boston, MA, USA

    Ulrich Müller

  4. Department of Pediatrics, Harvard Medical School, 02115, Boston, MA, USA

    Ulrich Müller

Authors
  1. Ulrich Lattermann
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  2. Ulrich Müller
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Lattermann, U., Müller, U. Immunoprecipitation of a male-specific polypeptide after in vitro translation of testicular poly(A)+ RNA. Hum Genet 79, 305–308 (1988). https://doi.org/10.1007/BF00282166

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  • DOI: https://doi.org/10.1007/BF00282166

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