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Reactive extraction of penicillin G in a pilot plant Karr-column

I. Model media

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Summary

Penicillin G was extracted from a model medium with a secondary amine (Amberlite LA-2) as carrier in n-butylacetate as solvent in a 7.6 m high pilot plant Karr-column at different stroke frequencies, throughput of the phases, concentrations of Penicillin G and carrier and ratios of the throughputs of the aqueous and organic phases. Up to penicillin concentrations of 30 gl−1, throughputs of the aqueous phase of 100 lh−1 and throughput ratios of the aqueous phase-to-organic phase of 3, very high degrees of extraction (99%) can be achieved with a penicillin loss below 1%.

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Abbreviations

a:

specific interfacial area with regard to the volume of the continuous phase

C:

partition coefficient

cA, cA, i:

concentration of carrier (sec. amine) in the bulk at the interface

cAHP, cAHP, i:

concentration of complex in the bulk at the interface

cH:

proton concentration

cHPa, cHPa,i :

concentration of free acid in the bulk of the aqueous phase at the interface

cHPo, cHPo, i :

concentration of free acid in the bulk of the organic phase, at the interface

cP, cP, i:

concentration of acid anions in the bulk of the aqueous phase, at the interface

d32 :

Sauter droplet diameter

E:

degree of extraction

f:

stroke frequency

KG:

reaction equilibrium constant

Kphys :

distribution coefficient

N:

number of stages in cascade

t:

mean residence time of the aqueous phase

\(\dot V\) aq :

throughput of the aqueous phase

\(\dot V\) o :

throughput of the organic phase

Z:

dimensionless longitudinal coordinate of the column with regard to its active length (4 m)

ɛ:

holdup of the organic phase

References

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Authors and Affiliations

  1. Gesellschaft für Biotechnologische Forschung mbH, Mascheroder Weg 1, D-3300, Braunschweig, Federal Republic of Germany

    B. Müller & L. Bischoff

  2. Lurgi GmbH, Gwinnerstr. 27/33, D-6000, Frankfurt 60, Federal Republic of Germany

    E. Schlichting

  3. Institut für Technische Chemie, Universität Hannover, Callinstr. 3, D-3000, Hannover, Federal Republic of Germany

    K. Schügerl

Authors
  1. B. Müller
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  2. E. Schlichting
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  3. L. Bischoff
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  4. K. Schügerl
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Müller, B., Schlichting, E., Bischoff, L. et al. Reactive extraction of penicillin G in a pilot plant Karr-column. Appl Microbiol Biotechnol 26, 36–41 (1987). https://doi.org/10.1007/BF00282146

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  • DOI: https://doi.org/10.1007/BF00282146

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