Summary
We have described a characteristic substructure of mitotic chromosomes, the chromosomal unit fibre, with lengths about five times the length of the corresponding metaphase chromosomes and a uniform diameter of 0.4 μm. In order to study the relationship of chromosome banding to chromosome compaction, methods have been devised to obtain banding patterns on chromosomal unit fibres, similar to G-band patterns of intact mitotic chromosomes. The total number of bands plus interbands per haploid human karyotype is estimated at about 3000. The banding pattern of chromosomal unit fibres indicates a certain resemblance to the normal G-banding pattern of human chromosomes even if the details indicate a short-range random distribution.
References
Bahr GF, Mikel U, Engler WF (1973) Correlates of chromosomal banding at the level of ultrastructure. In: Casperson T, Zech L. (eds) Chromosome identification. Nobel Symposium 23. Academic Press, New York, p 280
Bak AL, Zeuthen J, Crick FHC (1977) Higher-order structure of human mitotic chromosomes. Proc Natl Acad Sci USA 74: 1595–1599
Bak AL, Bak P, Zeuthen J (1979a) Higher levels of organization in chromosomes. J Theor Biol 76:205–217
Bak P, Bak AL, Zeuthen J (1979b) Characterization of human chromosomal unit fibres. Chromosoma 73:301–315
Comings DE (1978) Mechanisms of chromosome banding and implications for chromosome structure. Annu Rev Genet 12:25–46
Derr RJ (1977) Two-minute method for banding human chromosomes. Lancet 2:1141
Finch JT, Klug A (1976) Solenoidal model for superstructure in chromatin. Proc Natl Acad Sci USA 73:1897–1901
Hungerford DA, Hungerford AM (1978) Chromosome structure and function in man. VI. Pachytene chromomere maps of 16,17 and 18; pachytene as a reference standard for metaphase banding. Cytogenet Cell Genet 21:212–230
Kornberg RD (1977) Structure of chromatin. Annu Rev Biochem 46: 931–954
Ris H, Korenberg J (1979) Chromosome structure and levels of chromosome organization. In: Prescott DM, Goldstein L (eds) Cell biology, vol 2. Academic Press, New York, p 267
Ruzicka F (1973) Über die Primärwirkungen menschlicher Chromosomen. Hum Genet 20:335–341
Schwarzacher HG, Ruzicka F, Sperling K (1976) Electron microscopy of human banded and prematurely condensed chromosomes. In: Pearson PL, Lewis KR (eds) Chromosomes today, vol 5. Longman Group Ltd. Edinburgh, p 227
Yunis JJ (1976) High resolution of human chromosomes. Science 191: 1268–1270
Yunis JJ, Roldan L, Yasmineh WG, Lee JC (1971) Staining of satellite DNA in metaphase chromosomes. Nature 23: 532–533
Yunis JJ, Sawyer JR, Ball DW (1978) The characterization of high-resolution G-banded chromosomes of man. Chromosoma 67: 293–307
Yunis JJ, Ball DW, Sawyer JR (1979) G-banding patterns of high-resolution human chromosomes 6–22, X, and Y. Hum Genet 49: 291–306
Zeuthen J, Bak P, Bak AL (1979) Chromosomal unit fibers in Drosophila. Chromosoma 73:317–326
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Bak, A.L., Jørgensen, A.L. & Zeuthen, J. Chromosome banding and compaction. Hum Genet 57, 199–202 (1981). https://doi.org/10.1007/BF00282022
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DOI: https://doi.org/10.1007/BF00282022