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Phenotypic interaction studies of HPRT mutant and normal human fibroblasts

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Summary

Hypoxanthine incorporation was studied in growing HPRT mutant cells by preincubating them with extracts from normal cells, HPRT mutant cells, and extracts of their lyophilized cell sediment. HPRT mutant cells showed no increase of hypoxanthine uptake after preincubation with extracts of mutant cells, whereas after preincubation with extracts from normal cells and lyophilized sediment of HPRT mutant cells the incorporation rate was increased. This effect could not be observed when normal cells were preincubated with extracts of lyophilized sediments of normal cell lines.

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Authors and Affiliations

  1. Institute of Human Genetics, University of Hamburg, Butenfeld 32, D-2000, Hamburg 54, Federal Republic of Germany

    I. Willers, S. Singh & H. W. Goedde

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  1. I. Willers
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  2. S. Singh
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  3. H. W. Goedde
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Dedicated to Prof. H. Holzer on the occasion of his 60th birthday

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Willers, I., Singh, S. & Goedde, H.W. Phenotypic interaction studies of HPRT mutant and normal human fibroblasts. Hum Genet 57, 189–191 (1981). https://doi.org/10.1007/BF00282020

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  • DOI: https://doi.org/10.1007/BF00282020

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