Abstract
We have reported that Pisum sativum agglutinin (PSA), a plant lectin which recognizes mannosyl residues, accumulates markedly in Ehrlich solid tumor (EST) and suggested the possibility of applying PSA to tumor imaging radiopharmaceuticals. In the present work, an inflammation was induced by implantation of cotton thread in the left rear leg skeletal muscle of ddY mice and Ehrlich ascites tumor cells were inoculated into the right rear leg. 67Gacitrate accumulated in the tumor tissue and the inflammatory lesion to almost equal extents. On the other hand, 125IPSA preferentially accumulated in tumor tissues in mice bearing both tumor and inflammation. The results suggest that differential diagnosis of tumor from inflammation using radiolabeled PSA may be possible.
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References
Aizawa Y, Iwasaki M (1969) Influence of inflammation on the phospholipid metabolism in skeletal muscle of mouse and action of anti-inflammatory steroid. Yakugaku Zasshi 89:1664–1668
Boniface GR, Suresh MR, Willans DJ, Tam YK, Shysh A, Longenceker BM, Noujaim AA (1986) Renal tubular receptor imaging with iodine-131-labeled peanut lectin: Pharmacokinetics and renal clearance mechanism in animals. J Nucl Med 27:668–676
Edwards CL, Hayes RL (1969) Tumor scanning with 67Ga citrate. J Nucl Med 10:103–105
Hoffer P (1980) Gallium and infection. J Nucl Med 21:484–488
Inbar M, Rabinowitz Z, Sachs L (1969) The formation of variants with a reversion of properties of transformed cells. III Reversion of the structure of the cell surface membranne. Int J Cancer 4:690–696
Johnston GS (1981) Clinical application of gallium in oncology. Int J Nucl Med Biol 8:249–255
Kojima S, Hama Y, Miyashita K, Kubodera A (1982) Uptake of 67Ga in the liver of rats treated with CCl4. Kaku Igaku 19:67–74
Kojima S, Hama Y, Sasaki T, Kubodera A (1983) Elevated uptake of 67Ga and increased heparan sulfate content in liver-damaged-rats. Eur J Nucl Med 8:52–59
Kojima S, Kubodera A (1984) In vitro binding of 67Ga to Ehrlich ascites tumor cells. Eur J Nucl Med 9:374–378
Kojima S, Kubodera A (1985) Involvement of heparan sulfate in 67Ga binding to rat liver plasma membrane. Eur J Nucl Med 10:535–539
Kojima S (1986) Uptake of 67Ga citrate in tissues and heparan sulfate. Radioisotopes 35:347–445
Kojima S, Jay M (1986) Application of lectins to tumor imaging radiopharmaceuticals. Eur J Nucl Med 12:385–389
Kojima S, Jay M (1987) Comparisons of labeling efficiency, biological activity and biodistribution among 125I- 67Ga-DTPA- and 67Ga-DFO-lectins. Eur J Nucl Med 13: in press
Kubota K, Matsuzawa T, Fujiwara T, Abe Y, Hatazawa J, Kawabe H, Miyazawa H, Sato T, Ido T, Ishiwata K (1985) An experimental study on differential diagnosis of cancer from inflammation using 11C-L-methionine. Kaku Igaku 22:1327–1332
Louis CJ, Wyllie RG (1981) Fluorescein-concanavalin A conjugates distinguish between normal and malignant human cells: A preliminary report. Experientia 37:508–509
Ogihara I, Kojima S, Jay M (1986) Differential uptake of gallium-67-labeled liposomes between tumors and inflammatory lesions in rats. J Nucl Med 27:1300–1307
Sela BA, Lis H, Sachs L (1970) Different locations of carbohydrate-containing sites in the surface membrane of normal and transformed mammalian cells. J Membrane Biol 3:267–279
Shysh A, Eu SM, Noujaim AA, Suresh MR, Longenecker M (1985) In vivo localization of radioiodinated peanut lectin in a murine TA3/Ha mammary carcinoma model. Eur J Nucl Med 10:68–74
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Kojima, S., Jay, M. An experimental study on differential diagnosis of tumor from inflammation by using 125I labeled Pisum sativum agglutinin. Eur J Nucl Med 13, 474–477 (1987). https://doi.org/10.1007/BF00281863
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DOI: https://doi.org/10.1007/BF00281863