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The chromosomal distribution of repetitive DNA sequences within the human β globin gene cluster

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Summary

The chromosomal distribution and degree of repetitiveness of the sequences within the human β globin gene cluster has been studied by in situ hybridisation. A genomic recombinant, H\G1, which contains 15.9 thousand base pairs (kb) of DNA inserted into λ charon 4A, was used as a template for the [3H] complementary RNA used for hybridisation. The inserted DNA contains sequences 4.7 kb to the 5′ side of the δ gene and continues through the δ and β genes to a site 2.8 kb to the 3′ side of the β gene. Two highly repetitive sequences, which are distributed evenly over all the chromosomes, have been identified within this DNA. Another somewhat less repetitive sequence has been identified between the β and δ genes.

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Authors and Affiliations

  1. Department of Biochemistry, Queen Elizabeth College, University of London, Campden Hill Road, W8 7AH, London, UK

    S. Malcolm & P. Barton

  2. Institute of Medical Genetics, Yorkhill, Glasgow, UK

    S. Malcolm & M. A. Ferguson-Smith

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  1. S. Malcolm
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  2. P. Barton
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  3. M. A. Ferguson-Smith
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Malcolm, S., Barton, P. & Ferguson-Smith, M.A. The chromosomal distribution of repetitive DNA sequences within the human β globin gene cluster. Hum Genet 57, 388–393 (1981). https://doi.org/10.1007/BF00281691

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  • DOI: https://doi.org/10.1007/BF00281691

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