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A general suppressor of RNA polymerase I, II and III mutations in Saccharomyces cerevisiae

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Abstract

A multicopy genomic library of Saccharomyces cerevisiae (strain FL100) was screened for its ability to suppress conditionally defective mutations altering the 31 kDa subunit (rpc31–236) or the 53 kDa subunit (rpc53-254/424) of RNA polymerase III. In addition to allele-specific suppressors, we identified seven suppressor clones that acted on both mutations and also suppressed several other conditional mutations defective in RNA polymerases I or II. All these clones harbored a complete copy of the SSD1 gene. The same pleiotropic suppression pattern was found with the dominant SSD1-v allele present in some laboratory strains of S. cerevisiae. SSD1-v was previously shown to suppress mutations defective in the SIT4 gene product (a predicted protein phosphatase subunit) or in the regulatory subunit of the cyclic AMP-dependent protein kinase. We propose that the SSD1 gene product modulates the activity (or the level) of the three nuclear RNA polymerases, possibly by altering their degree of phosphorylation.

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Authors and Affiliations

  1. Service de Biochimie et Génétique Moléculaire, Département de Biologie Cellulaire et Moléculaire, C.E.A. Centre d'Etudes de Saclay, Bat. 142, F-91191, (Cedex) Gif sur Yvette, France

    Sophie Stettler, Nuchanard Chiannilkulchai, Sylvie Hermann-Le Denmat, Dominique Lalo, André Sentenac & Pierre Thuriaux

  2. Centre de Génétique Moléculaire du C.N.R.S., Laboratoire associé a l'Université Pierre et Marie Curie, France

    François Lacroute

Authors
  1. Sophie Stettler
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  2. Nuchanard Chiannilkulchai
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  3. Sylvie Hermann-Le Denmat
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  4. Dominique Lalo
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  5. François Lacroute
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  6. André Sentenac
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  7. Pierre Thuriaux
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Communicated by C.P. Hollenberg

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Stettler, S., Chiannilkulchai, N., Hermann-Le Denmat, S. et al. A general suppressor of RNA polymerase I, II and III mutations in Saccharomyces cerevisiae . Molec. Gen. Genet. 239, 169–176 (1993). https://doi.org/10.1007/BF00281615

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  • DOI: https://doi.org/10.1007/BF00281615

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