Summary
The human Y chromosome can be differentially stained with Giemsa using simple procedures. This phenomenon is strikingly similar to that observed with quinacrine fluorescence.
The specific Giemsa-Y stain may be selectively removed by the action of an oil. The same oil, under certain conditions, selectively removes Giemsa stain from all chromosomes, resulting in R- and T-banding patterns. These bands, which are obtained through subtraction of dye from Giemsa-stained chromosomes, allow slides to be further processed.
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References
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Dallapiccola, B., Ricci, N. Observations on specific Giemsa staining of the Y and on selective oil destaining of the chromosomes. Hum Genet 26, 251–255 (1975). https://doi.org/10.1007/BF00281461
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DOI: https://doi.org/10.1007/BF00281461