Summary
Twenty-seven late larval or early pupal lethal mutations were isolated for the X-chromosome, some of which showed structural and/or functional deficiencies of the imaginal discs. The mutants were grouped according to the size and morphology of their discs as follows: 1. discs normal: 18 mutants. 2. discs small: 2 mutants. 3. discs degenerate: 4 mutants. 4. discless: 1 mutant. 5. discs heterogeneous: 2 mutants. Preliminary characterization of the mutants included a study of disc morphology, puparium formation and pupal molt, in vivo and in vitro evagination of the imaginal discs, autonomy of the mutation in the disc tissue (differentiation after transplantation and gynander mosaicism test). Possible relations between disc morphology and the former characteristics are discussed.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Literature
Brown, S.W.; Hannah, A.: An induced maternal effect on the stability of the ring X-chromosome of Drosophila melanogaster. Proc. Nat. Acad. Sci. U.S. 38, 687–693 (1952)
Bryant, P.J.; Zornetzer, M.: Mosaic analysis of lethal mutations in Drosophila. Genetics 75, 623–637 (1973)
Ephrussi, B.; Beadle, G.W.: A technique of transplantation for Drosophila. Amer. Natur. 70, 218–225 (1936)
Fristrom, D.; Fristrom, J.W.: The mechanism of evagination of imaginal discs of Drosophila melanogaster. Develop. Biol. 43, 1–23 (1975)
Fristrom, J.W.: The biochemistry of imaginal disc development. In: “The Biology of Imaginal Discs” (Eds. Ursprung, H.; Nöthiger, R.), series Results and Problems in Cell Differentiation, Vol. 5, 109–154. Berlin, Heidelberg, New York: Sprin-ger 1972
Fristrom, J.W.; Logan, W.R.; Murphy, C.: The synthetic and minimal culture requirements for evagination of imaginal discs in Drosophila melanogaster in vitro. Develop. Biol. 33, 441–456 (1973)
Fristrom, J.W.; Raikow, R.; Petri, W.; Stewart, D.: In vitro evagination and RNA synthesis in imaginal discs of Drosophila melanogaster. In: RNA in Development (ed. Hanley, E.W.), Park City Symposium on Problems in Biology, 381–401. Salt Lake City, Utah: University of Utah Press 1970
Hadorn, E.: Developmental genetics and lethal factors. New York: John Wiley and Sons, Inc. 1961
Hinton, C.W.: The behavior of an unstable ring chromosome of Drosophila melanogaster. Genetics 40, 951–961 (1955)
Jizba, J.; Herout, V.: Plant substances XXVI. Isolation of constituents of common polypody rhizomes (Polypodium vulgare L.). Collection of Czechoslovak Chemical Communications 32, 2867–2874 (1967)
Lewis, F.B.; Bacher, F.: Method of feeding ethyl methane sulfonate (EMS) to Drosophila males. Dros. Inf. Serv. 43, 193 (1968)
Lindsley, D.L.; Grell, E.H.: Genetic variations of Drosophila melanogaster. Carnegie Inst. Wash. Publ. 627 (1968)
Mandaron, P.: Sur le mécanisme de l'evagination des disques imagineux chez la Drosophile. Develop. Biol. 25, 581–605 (1971)
Murphy, C.: Cell death and autonomous gene action in lethals effecting imaginal dises in Drosophila melanogaster. Develop. Biol. 39, 23–36 (1974)
Nöthiger, R.: The larval development of imaginal discs. In: “The Biology of Imaginal Discs” (Eds. Ursprung, H.; Nöthiger, R.), series: Results and Problems in Cell Differentiation, Vol. 5, 1–34. Berlin, Heidelberg, New York: Springer 1972
Poodry, C.; Schneiderman, H.A.: The ultrasctructure of the developing leg of Drosophila melanogaster. Wilhelm Roux' Arch. Entwicklungsmech. d. Organismen 166, 1–44 (1970)
Postlethwait, J.H.; Schneiderman, H.A.: Developmental genetics of Drosophila imaginal discs. Ann. Rev. Genet. 7, 381–433 (1973)
Rice, T.B.: Isolation and characterization of maternal-effect mutants: an approach to the study of early determination in Drosophila melanogaster. Ph. D. Dissertation, Yale University (1973)
Rice, T.B.; Garen, A.: Localized defects of blastoderm formation in maternal effect mutants of Drosophila. Develop. Biol. 43, 277–286 (1975)
Robb, J.A.: Maintenance of imaginal discs of Drosophila melanogaster in chemically defined media. J. Cell Biol. 41, 876–885 (1969)
Russell, M.A.: Pattern formation in the imaginal discs of a temperature-sensitive cell-lethal mutant of Drosophila melanogaster. Develop. Biol. 40, 24–39 (1974)
Shearn, A.: Complementation analysis of late lethal mutants of Drosophila melanogaster. Genetics 77, 115–125 (1974)
Shearn, A.; Garen, A.: Genetic control of imaginal disc development in Drosophila. Proc. Nat. Acad. Sci. U.S. 71, 1393–1397 (1974)
Shearn, A.; Rice, T.; Garen, A.; Gehring, W.: Imaginal disc abnormalities in lethal mutants of Drosophila. Proc. Nat. Acad. Sci. U.S. 68, 2594–2598 (1971)
Stern, C.; Tokunaga, C.: Autonomous pleiotropy in Drosophila. Proc. Nat. Acad. Sci. U.S. 60, 1252–1259 (1968)
Spreij, T.H.: Cell death during the development of the imaginal discs of Calliphora erythrocephala. Neth. J. Zool. 21, 221–264 (1971)
Stewart, M.; Murphy, C.; Fristrom, J.W.: The recovery and preliminary characterization of X-chromosome mutants affecting imaginal discs of Drosophila melanogaster. Develop. Biol. 27, 71–83 (1972)
Ursprung, H.: The fine structure of imaginal discs. In: “The Biology of Imaginal Discs” (Eds. Ursprung, H.; Nöthiger, R.), series: Results and Problems in Cell Differentiation Vol. 5, 93–107. Berlin, Heidelberg, New York: Springer 1972
Ursprung, H.; Nöthiger, R.: The Biology of Imaginal Discs. Results and Problems in Cell Differentiation, Vol. 5. Berlin, Heidelberg, New York: Springer 1972
Author information
Authors and Affiliations
Additional information
Communicated by L. Alföldi
Rights and permissions
About this article
Cite this article
Kiss, I., Bencze, G., Fekete, É. et al. Isolation and characterization of X-linked lethal mutants affecting differentiation of the imaginal discs in Drosophila melanogaster . Theoret. Appl. Genetics 48, 217–226 (1976). https://doi.org/10.1007/BF00281284
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00281284