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Studies on the mechanism of chromosome banding with trypsin

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Summary

Human chromosomes were treated with either trypsin or specifically inhibited trypsin and stained with Leishman stain. Bands were only obtained after trypsin treatment. This may indicate that binding-specific proteolytic activity was essential for the production of bands.

Zusammenfassung

Menschliche Chromosomen wurden entweder mit Trypsin oder mit spezifisch gehemmtem Trypsin behandelt und nach Leishman gefärbt. Bandenmuster wurden nur nach Trypsin-behandlung gesehen; das kann bedeuten, daß die bindungsspezifische protolytische Aktivität für das Auftreten von Banden entscheidend ist.

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Authors and Affiliations

  1. Division of Teratology, Department of Obstetrics and Gynaecology, Rigshospitalet, University of Copenhagen, Copenhagen

    C. Lundsteen, Lene Kristoffersen & F. Ottosen

  2. Department of Paediatrics, Rigshospitalet, University of Copenhagen, Copenhagen

    C. Lundsteen, Lene Kristoffersen & F. Ottosen

Authors
  1. C. Lundsteen
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  2. Lene Kristoffersen
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  3. F. Ottosen
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Additional information

Supported by the Danish Medical Research Council and the Danish Cystic Fibrosis Foundation.

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Lundsteen, C., Kristoffersen, L. & Ottosen, F. Studies on the mechanism of chromosome banding with trypsin. Hum Genet 24, 67–69 (1974). https://doi.org/10.1007/BF00281110

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  • DOI: https://doi.org/10.1007/BF00281110

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