Summary
In Chinese hamster-human hybrids with overlapping translocations, the major site of hybridization of a cDNA clone for the liver form of human α-l-fucosidase was 1p36.3→1p34, consistent with hybridization to the FUCA1 locus. No hybridization to the FUCA2 locus on chromosome 6 was observed. Hybridization to a genomic sequence on chromosome 2 was, however, detected, thus defining a new FUCA-like locus. The restriction map of the α-fucosidase cDNA could be exactly superimposed upon its region of homology within a genomic clone containing this FUCA-like locus, suggesting that it is a processed pseudogene.
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References
Carritt B, Goldfarb PSG, Hooper ML, Slack C (1977) Chromosome assignment of a human gene for argininosuccinate synthetase expression in human x Chinese hamster somatic cell hybrids. Exp Cell Res 106:71–78
Carritt B, King J, Welch HM (1982) Gene order and localization of enzyme loci on the short arm of chromosome 1. Ann Hum Genet 46:329–335
Carritt B, Welch HM, Parry-Jones NJ (1986) Sequences homologous to the human D1S1 locus present on human chromosome 3. Am J Hum Genet 38:428–436
Corney G, Fisher RA, Cook PJL, Noades J, Robson EB (1977) Linkage between α-l-fucosidase and the Rh blood group. Ann Hum Genet 40:403–405
Eiberg H, Mohr J (1984) Synteny of plasma α-l-fucosidase and the plasminogen system. (7th International Workshop on Human Gene Mapping) Cytogenet Cell Genet 37:460
Fukushima H, de Wet JR, O'Brien JS (1985) Molecular cloning of a cDNA for human α-l-fucosidase. Proc Natl Acad Sci USA 82: 1262–1265
Humphries P, Barton D, McKay A, Humphries MM, Carritt B (1983) Isolation of a polymorphic DNA segment unique to human chromosome 7 by molecular cloning of hybrid cell DNA. MGG 190: 143–149
Lawn RM, Fritsch EF, Parker RC, Blake G, Maniatis T (1978) The isolation and characterization of a linked-and β-globin gene from a cloned library of human DNA. Cell 15:1157–1174
Murray JC, Sadler E, Eddy RL, Shows TB, Buetow KH (1985) Evidence for assignment of plasminogen to chromosome 6, not chromosome 4. (8th International Workshop on Human Gene Mapping) Cytogenet Cell Genet 40:709
Ng WG, Donnell GN, Koch R, Bergen WR (1976) Biochemical and genetic studies of plasma and leukocyte α-l-fucosidase. Am J Hum Genet 28:42–50
Turner BM, Turner VS, Beratis NG, Hirschhorn K (1975) Polymorphism of human α-l-fucosidase. Am J Hum Genet 27:651–661
Turner BM, Smith M, Turner VS, Kucherlapati RS, Ruddle FH, Hirschhorn K (1976) Assignment of the gene locus for human α-l-fucosidase to chromosome 1 by analysis of somatic cell hybrids. Somatic Cell Genet 4:45–54
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Carritt, B., Welch, H.M. An α-fucosidase pseudogene on human chromosome 2. Hum Genet 75, 248–250 (1987). https://doi.org/10.1007/BF00281068
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DOI: https://doi.org/10.1007/BF00281068