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Asymmetric fusion between protoplasts of tomato (Lycopersicon esculentum Mill.) and gamma-irradiated protoplasts of potato (Solanum tuberosum L.): the effects of gamma irradiation

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Abstract

This paper describes the aggregation of nuclei in heterokaryons of tomato and unirradiated or irradiated potato protoplasts and the effects of gamma irradiation of potato and tomato protoplasts on single- and double-stranded DNA fragmentation, DNA repair and DNA synthesis as revealed by alkaline and pulsed field gel electrophoresis and an immunocytochemical technique. The prospects for obtaining highly asymmetric somatic hybrids of tomato and gamma-irradiated potato are discussed.

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Authors and Affiliations

  1. Department of Genetics, Wageningen Agricultural University, Dreijenlaan 2, NL-6703 HA, Wageningen, The Netherlands

    Herman C. H. Schoenmakers, José J. M. van der Meulen-Muisers & Maarten Koornneef

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  1. Herman C. H. Schoenmakers
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  2. José J. M. van der Meulen-Muisers
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  3. Maarten Koornneef
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Communicated by R. Hagemann

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Schoenmakers, H.C.H., van der Meulen-Muisers, J.J.M. & Koornneef, M. Asymmetric fusion between protoplasts of tomato (Lycopersicon esculentum Mill.) and gamma-irradiated protoplasts of potato (Solanum tuberosum L.): the effects of gamma irradiation. Molec. Gen. Genet. 242, 313–320 (1994). https://doi.org/10.1007/BF00280421

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  • DOI: https://doi.org/10.1007/BF00280421

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