Summary
Oenothera plants homozygous for a recessive allele at the plastome mutator (pm) locus show non-Mendelian mutation frequencies that are 1000-fold higher than spontaneous levels. Chloroplast DNA (cpDNA) was isolated from nine mutants and two green isolates of the plastome mutator line. cpDNA restriction patterns were compared to cpDNA from a representative of the progenitor Johansen strain, and cpDNAs from all eleven plastome mutator lines show changes of fragment mobility due to deletion events at five discrete regions of the plastome. Most of the mutants have cpDNA restriction patterns identical to that of one of the green isolates from the plastome mutator line, and therefore, most of the differences in fragment length are probably not responsible for the mutant phenotypes. In contrast to the plastome mutator line, cpDNA from several populations of a closely related wild-type Oenothera species have few restriction fragment length polymorphisms. This suggests that both mutation frequencies and site-specific cpDNA deletions are elevated in the plastome mutator line, and implicates a defect in the cpDNA repair or replication machinery.
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References
Blasko K, Kaplan SA, Higgins KG, Wolfson R, Sears BB (1988) Variation in copy number of a 24-base pair tandem repeat in the chloroplast DNA of Oenothera hookeri strain Johansen. Curr Genet 14:287–292
Charlebois RL, Doolittle WF (1989) Transposable elements and genome structure in halobacteria. In: Berg DE, Howe MM (eds) Mobile DNA. Am Soc Microbiol, pp 297–307
Chiu W-L, Sears BB (1985) Recombination between chloroplast DNAs does not occur in sexual crosses of Oenothera. Mot Gen Genet 198:525–528
Chiu W-L, Stubbe W, Sears BB (1988) Plastid inheritance in Oenothera: organelle genome modifies the extent of biparental plastid transmission. Curr Genet 13:181–189
Cleland RE (1935) Cyto-taxonomic studies on certain Oenotheras from California. Proc Am Phil Soc 75:339–429
Cleland RE (1972) Oenothera. Academic Press, NY, p 370
Curtis SE, Clegg MT (1984) Molecular evolution of chloroplast DNA sequences. Mot Biol Evol 1:291–301
Doebley JF, Ma DP, Renfroe WT (1987) Insertion/deletion mutations in the Zea chloroplast genome. Curr Genet 11:617–624
Epp MD (1973) Nuclear gene-induced plastome mutations in Oenothera hookeri. I. Genetic analysis. Genetics 75:465–483
Epp MD, Parthasarthy MV (1987) Nuclear gene-plastome mutations in Oenothera hookeri. II. Phenotype description with electron microscopy. Am J Bot 74:143–151
Epp MD, Erion JL, Sears BB, Stubbe W (1987) The plastome imitator of Oenothera continues to function as originally described. Mol Gen Genet 206:515–518
Gordon KHJ, Crouse EJ, Bohnert HJ, Herrmann RG (1982) Physical mapping of differences in chloroplast DNA of the five wild-type plastomes in Oenothera subsection Euoenothera. Theor Appl Genet 61:281–296
Herrmann RG, Westoff P, Alt J, Winter P, Tittgen J, Bisanz C, Sears BB, Nelson N, Hurt E, Hauska G, Viebrock A, Sebald W (1983) Identification and characterization of genes for polypeptides of the thylakoid membrane. In: Cifferi O, Dure L III (eds) Structure and function of plant genomes. Plenum, New York, pp 143–153
Hudspeth MES, Shumard DS, Tatti KH, Grossman LI (1980) Rapid purification of yeast mitochondrial DNA in high yield. Biochim Biophys Acta 610:221–228
Johnson EM (1988) Physiological and molecular characterizations of plastids from a plastome mutator-induced mutant of Oenothera hookeri strain Johansen. Ph. D. Thesis, Michigan State University
Johnson EM, Sears BB (1990) Membrane composition and physiological activity of plastids from an Oenothera plastome mutator-induced hloroplast mutant. Plant Physiol 91, in press
Kutzelnigg H, Stubbe W (1974) Investigations on plastome mutants in Oenothera. I. General considerations. Sub-Cell Biochem 3:73–89
Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning. A laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, p 545
Michaelis P (1969) Über Plastiden-Restitutionen (Ruckmutationen). Cytologia 34 (Suppl):1–115
Palmer JD (1985) Evolution of chloroplast and mitochondrial DNA in plants and algae. In: MacIntyre RJ (ed) Molecular evolutionary genetics. Plenum, New York, pp 131–239
Potrykus I (1970) Mutation und Ruckmutation extrachromosomale vererbter Plastidenmerkmale von Petunia. Z Pflanzenzuch 63:24–40
Raven PH, Dietrich W, Stubbe W (1979) An outline of the systematics of Oenothera subsect. Euoenothera (Onagraceae). Syst Bot 4:242–252
Redei GP, Plurad SB (1973) Hereditary structural alterations of plastids induced by a nuclear gene in Arabidopsis. Protoplasma 77:361–380
Salts Y, Herrmann RG, Peleg N, Lavi U, Izhar S, Frankel R, Beckmann JS (1984) Physical mapping of plastid DNA variation among eleven Nicotiana species. Theor Appl Genet 69:1–14
Schmitz UK, Kowallik KV (1986) Polymorphism and gene arrangement among plastomes of ten Epilobium species. Plant Mol Biol 7:115–127
Sears BB (1983) Genetics and evolution of the chloroplast. In: Gustafsson P (ed) Stadler Symposium XV, University of Missouri Press, Columbia, Mo, pp 119–139
Shumway LK, Weier TE (1967) The chloroplast structure of iojap maize. Am J Bot 54:773–780
Stein J vom, Hachtel W (1986) Chloroplast DNA differences between two species of Oenothera subsection Munzia: location in the chloroplast genome and relevance to possible interactions between nuclear and plastid genomes. Theor Appl Genet 73:141–147
Stein J vom, Hachtel W (1988a) Chloroplast DNA differences in the genus Oenothera subsection Munzia: a short direct repeat resembling the lambda chromosomal attachment site occurs as a deletion/insertion within an intron of an NADH-dehydrogenase gene. Curr Genet 13:191–197
Stein J vom, Hachtel W (1988b) Deletions/insertions, short inverted repeats, sequences resembling att-lambda, and frame shift mutated open reading frames are involved in chloroplast DNA differences in the genus Oenothera subsection Munzia. Mol Gen Genet 213:513–518
Steiner EE (1951) Phylogenetic relationships of certain races of Euoenothera from Mexico and Guatemala. Evolution 5:265–272
Stubbe W (1959) Genetische Analyse des Zusammenwirken von Genom und Plastom bei Oenothera. Z Bot 48:191–218
Stubbe W, Herrmann RG (1982) Selection and maintenance of plastome mutants and interspecific genome/plastome hybrids from Oenothera. In: Edelman M, Hallick RB, Chua N-H (eds) Methods in chloroplast molecular biology. Elsevier, Amsterdam, pp 149–165
Thompson D, Walbot V, Coe EH Jr (1983) Plastid development in iojap and mutator-affected maize plants. Am J Bot 70:940–950
Walbot V, Coe EH Jr (1979) Nuclear gene iojap conditions a programmed change to ribosome-less plastids in Zea mays. Proc Natl Acad Sci USA 76:2760–2764
Zurawski G, Clegg MT (1987) Evolution of higher plant chloroplast DNA-encoded genes: implications for structure-function and phylogenetic studies. Annu Rev Plant Physiol 38:391–418
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Communicated by G.R. Fink
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Chiu, WL., Johnson, E.M., Kaplan, S.A. et al. Oenothera chloroplast DNA polymorphisms associated with plastome mutator activity. Molec. Gen. Genet. 221, 59–64 (1990). https://doi.org/10.1007/BF00280368
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DOI: https://doi.org/10.1007/BF00280368