Summary
The molecular cloning and nucleotide sequence of elements from potato and pepper that are related to the recently identified Tst1 element are described. Sequence analysis reveals considerable conservation of sequences internal to both the Tst1 element and two of the related elements identified here. In six potato clones analysed, the II by inverted repeat first identified in the Tst1 element is conserved. Several of the elements are flanked by an 8 by direct repeat. DNA fragments which were amplified from several pepper genomes by polymerase chain reaction (PCR) amplification using the inverted repeat as sequence primers also display considerable conservation of sequences internal to the Tst1 element. These data further support the possibility that Tst1 is a non-autonomous transposable element and that Tst1 might be the first example of a transposable element which occurs in several genera of solanaceous plants.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bhattacharyya MK, Smith AM, Noel Ellis TH, Hedley C, Martin C (1990) The wrinkled-seed character of pea described by Mendel is caused by a transposon-like insertion in a gene encoding starch-branching enzyme. Cell 60:115–122
Döring HP (1989) Tagging genes with maize transposable elements. An overview. Maydica 34:73–88
Herrmann A, Schulz W, Hahlbrock K (1988) Two alleles of the single-copy chalcone synthase gene in parsley differ by a transposon-like element. Mol Gen Genet 212:93–98
Köster-Töpfer M, Frommer WB, Rocha-Sosa M, Willmitzer L (1990) Presence of a transposon-like element in the promoter region of an inactive patatin gene in Solanum tuberosum L. Plant Molecular Biology 14:239–247
Sanger F, Nicklen S, Coulsen AR (1977) DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA 74:5463–5467
Shirsat AH (1988) A transposon-like structure in the 5′ flanking sequence of a legumin gene from Pisum sativum. Mol Gen Genet 212:129–133
Southern E (1975) Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol 98:503–517
Spell ML, Baran G, Wessler SR (1988) An RFLP adjacent to the maize waxy gene has the structure of a transposable element. Mol Gen Genet 211:364–366
Vodkin LO, Rhodes PR, Goldberg RB (1983) cA lectin gene insertion has the structural features of a transposable element. Cell 34:1023–1031
Wienand U, Saedler H (1987) Plant transposable elements: unique structures for gene tagging and gene cloning. In: Hohn T, Schell J (eds) Plant DNA infectious agents; Plant gene research. Springer Verlag, Wien, New York, pp 205–227
Author information
Authors and Affiliations
Additional information
Communicated by H. Saedler
Rights and permissions
About this article
Cite this article
Kikuchi, S., Liu, X., Frommer, W.B. et al. Identification and structural characterization of further DNA elements in the potato and pepper genomes homologous to the transposable element-like insertion Tst1 . Molec. Gen. Genet. 230, 494–498 (1991). https://doi.org/10.1007/BF00280308
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00280308