Summary
Two classes of lipoxygenase (LOX) cDNAs, designated loxA and loxB, were isolated from soybean. A third lipoxygenase cDNA, loxP1, was isolated from pea. The deduced amino acid sequences of loxA and loxB show 61–74% identity with those of soybean seed LOXs. loxA and loxB mRNAs are abundant in roots and non-growing regions of seedling hypocotyls. Lower levels of these mRNAs are found in hypocotyl growing regions. Exposure of soybean seedlings to water deficit causes a rapid increase in loxA and loxB mRNAs in the elongating hypocotyl region. Similarly, loxP1 mRNA levels increase rapidly when pea plants are wilted. loxA and loxB mRNA levels also increase in wounded soybean leaves, and these mRNAs accumulate in soybean suspension cultures treated with 20 μM methyl jasmonate. These results demonstrate that LOX gene expression is modulated in response to water deficit and wounding and suggest a role for lipoxygenase in plant responses to these stresses.
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References
Bensen RJ, Boyer JS, Mullet JE (1988) Water deficit-induced changes in abscisic acid, growth, polysomes, and translatable RNA in soybean hypocotyls. Plant Physiol 88:289–294
Creelman RA, Mullet JE (1991) Water deficit modulates gene expression in growing zones of soybean seedlings. Analysis of differentially expressed cDNAs, a new β-tubulin gene, and expression of genes encoding cell wall proteins. Plant Mol Biol, in press
Creelman RA, Mason HS, Bensen RJ, Boyer JS, Mullet JE (1990) Water deficit and abscisic acid cause differential inhibition of shoot versus root growth in soybean seedlings. Plant Physiol 92:205–214
Ealing PM, Casey R (1988) The complete amino acid sequence of a pea (Pisum sativum) seed lipoxygenase predicted from a near full-length cDNA. Biochem J 253:915–918
Farmer EE, Ryan CA (1990) Interplant communication: airborne methyl jasmonate induces synthesis of proteinase inhibitors in plant leaves. Proc Nail Acad Sci USA 87:7713–7716
Firn RD, Friend J (1972) Enzymatic production of the plant growth inhibitor, xanthoxin. Planta 103:263–266
Graham JS, Hall G, Pearce G, Ryan CA (1986) Regulation of synthesis of proteinase inhibitors I and II mRNAs in leaves of wounded tomato plants. Planta 169:399–405
Guerrero FD, Mullet JE (1986) Increased abscisic acid biosynthesis during plant dehydration requires transcription. Plant Physiol 80:588–591
Guerrero FD, Mullet JE (1988) Reduction of turgor induces rapid changes in leaf translatable RNA. Plant Physiol 88:401–408
Hildebrand DF, Hamilton-Kemp TR, Legg CS, Bookjans G (1988) Plant lipoxygenases: occurrence, properties and possible functions. Curr Top Plant Biochem Physiol 7:201–219
Jin DF, West CA (1984) Characteristics of galacturonic acid oligomers as elicitors of casbene synthetase activity in castor bean seedlings. Plant Physiol 74:989–992
Mason HS, Mullet JE (1990) Expression of two soybean vegetative storage protein genes during development and in response to water deficit, wounding and jasmonic acid. Plant Cell 2:569–579
Mason HS, Guerrero FD, Boyer JS, Mullet JE (1988) Proteins homologous to leaf glycoproteins are abundant in stems of dark-grown soybean seedlings. Analysis of proteins and cDNAs. Plant Mol Biol 11:845–856
Needleman P, Turk J, Jakschik BA, Morrison AR, Lefkowith JB (1986) Arachidonic acid metabolism. Annu Rev Biochem 55:69–102
Ocampo CA, Moerschbacher B, Grambow HJ (1986) Increased lipoxygenase activity is involved in the hypersensitive response of wheat leaf cells infected with avirulent rust fungi or treated with fungal elicitor. Z Naturforsch 41:559–563
Park TK, Polacco JC (1989) Distinct lipoxygenase species appear in the hypocotyl/radicle of germinating soybean. Plant Physiol 90:285–290
Quarrie SA, Lister PG (1984) Effects of inhibitors of protein synthesis on abscisic acid accumulation in wheat. Z Pflanzenphysiol 114:309–314
Shibata D, Steczko J, Dixon JE, Hermodson M, Yazdanparast R, Axelrod B (1987) Primary structure of soybean lipoxygenase-1. J Biol Chem 262:10080–10085
Shibata D, Steczko J, Dixon JE, Andrews PC, Hermodson M, Axelrod B (1988) Primary structure of soybean lipoxygenase L-2. J Biol Chem 263:6816–6821
Shibata D, Kato T, Tanaka K (1991) Nucleotide sequences of a soybean lipoxygenase gene and the short intergenic region between an upstream lipoxygenase gene. Plant Mol Biol 16:353–359
Sindhu R, Walton DC (1987) The conversion of xanthoxin to abscisic acid by cell-free preparations from bean leaves. Plant Physiol 85:916–921
Takeo T, Tsushida T (1980) Changes in lipoxygenase activity in relation to lipid degradation in plucked tea shoots. Phytochemistry 19:2521–2522
Vick BA, Zimmerman DC (1983) The biosynthesis of jasmonic acid: a physiological role for plant lipoxygenase. Biochem Biophys Res Commun 111:470–477
Vick BA, Zimmerman DC (1987) Oxidative systems for modification of fatty acids: The lipoxygenase pathway. In: Stumpf PK (ed) The Biochemistry of Plants: A Comprehensive Treatise, vol 9. Academic Press, New York, pp 53–90
Wingate VPM, Lawton MA, Lamb CJ (1988) Glutathione causes a massive and selective induction of plant defense genes. Plant Physiol 87:206–210
Yenofsky RL, Fine M, Liu C (1988) Isolation and characterization of a soybean (Glycine max) lipoxygenase-3 gene. Mol Gen Genet 211:215–222
Zimmerman DC, Coudron CA (1979) Identification of traumatin, a wound hormone, as 12-oxo-trans-l0-dodecenoic acid. Plant Physiol 63:536–541
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Communicated by E. Meyerowitz
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Bell, E., Mullet, J.E. Lipoxygenase gene expression is modulated in plants by water deficit, wounding, and methyl jasmonate. Molec. Gen. Genet. 230, 456–462 (1991). https://doi.org/10.1007/BF00280303
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DOI: https://doi.org/10.1007/BF00280303