Summary
The product of the uaY gene of Aspergillus nidulans is necessary for the expression of at least eight genes coding for enzymes and permeases of the purine utilisation pathway. A detailed fine structure map has been constructed of this gene involving 13 presumed point mutations and eight deletions. Gene conversion of these deletions was demonstrated. A technique was devised to select for constitutive mutations and two were obtained which map within the uaY gene. We have shown that the most centromere proximal allele reverts to a number of different phenotypes. The properties of this allele suggest that it may map in the open reading frame of the uaY gene, in a domain that could be altered in a way that would differentially affect the expression of genes under uaY control.
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Alderson T, Scazzocchio C (1967) A system for the study of interlocus specificity for both forward and reverse mutation in at least eight gene loci in Aspergillus nidulans. Mutat Res 8:255–264
Darlington AJ, Scazzocchio C (1967) Use of analogues and the substrate sensitivity of mutants in analysis of purine uptake and breakdown in Aspergillus nidulans. J Bacteriol 93:937–940
Darlington AJ, Scazzocchio C, Pateman JA (1965) Biochemical and genetical studies of purine degradation in Aspergillus. Nature 206:599–600
Diallinas G, Scazzocchio C (1989) A gene coding for the uric acidxanthine permease of Aspergillus nidulans: Inactivational cloning, characterisation and sequence of a cis-acting mutation. Genetics 122:341–350
Greever RF, Huiet L, Baum JA, Tyler BM, Patel VB, Rutledge BJ, Case ME, Giles NH (1989) DNA sequence, organization and regulation of the qa gene cluster of Neurospora crassa. J Mol Biol 207:15–34
Hartley MJ (1969) Mutation in Aspergillus. PhD Thesis, University of Cambridge
Hynes MJ (1979) Fine structure mapping of the acetamidase structural gene and its controlling region in Aspergillus nidulans. Genetics 91:381–392
Johnstone M (1987) A model fungal regulatory mechanism, the GAL genes of Saccharomyces cerevisiae. Microbiol Rev 51:458–476
Käfer E (1958) An 8-chromosome map of Aspergillus nidulans. Adv Genet 9:105–145
Kudla B, Caddick MX, Langdon T, Martinez-Rossi N, Bennett CF, Sibley S, Davies RW, Arst Jr HN (1990) The regulatory gene areA mediating nitrogen metabolite repression in Aspergillus nidulans. Mutations affecting specificity of gene activation alter a loop residue of a putative zinc finger. EMBO J 9:1355–1364
Marczak JE, Brandriss MC (1991) Analysis of constitutive and noninducible mutations of the PUTS transcriptional activator. Mol Cell Biol 11:2609–2619
Phillipides D, Scazzocchio C (1981) Positive regulation in a eukaryote, a study of the uaY gene of Aspergillus nidulans. II. Identification of the effector binding protein. Mol Gen Genet 181:107–115
Scazzocchio C (1966) Studies on the genetic control of purine oxidation in Aspergillus nidulans. PhD Thesis, University of Cambridge
Scazzocchio C (1973) The genetic control of molybdoflavoproteins in Aspergillus nidulans. I: Use of the NADH dehydrogenase activity associated with xanthine dehydrogenase to investigate substrate and product induction. Mol Gen Genet 125:147–155
Scazzocchio C, Darlington AJ (1968) The induction and repression of the enzymes of purine breakdown in Apsergillus nidulans. Biochim Biophys Acta 166:557–568
Scazzocchio C, Holl FB, Foguelman AI (1973) The genetic control of molybdoflavoproteins in Aspergillus nidulans: Allopurinol-resistant mutants constitutive for xanthine dehydrogenase. Eur J Biochem 36:428–445
Scazzocchio C, Sdrin CN, Ong G (1982) Positive regulation in a eukaryote, a study of the uaY gene of Aspergillus nidulans: I. Characterization of alleles, dominance and complementation studies and a fine structure map of the uaY-oxpA cluster. Genetics 100:185–208
Sealy-Lewis HM, Scazzocchio C, Lee S (1978) A mutation efective in the xanthine alternative pathway of Aspergillus nidulans: Its use to investigate the specificity of uaY-mediated induction. Mol Gen Genet 164:303–308
Suarez T, Oestreicher N, Peñalva MA, Scazzocchio C (1991) Molecular cloning of the uaY regulatory gene of Aspergillus nidulans reveals a favoured region for DNA insertions. Mol Gen Genet
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Suárez, T., Oestreicher, N., Kelly, J. et al. The uaY positive control gene of Aspergillus nidulans: fine structure, isolation of constitutive mutants and reversion patterns. Molec. Gen. Genet. 230, 359–368 (1991). https://doi.org/10.1007/BF00280292
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DOI: https://doi.org/10.1007/BF00280292