Summary
Three phenotypical variants of normal human serum α2-macroglobulin revealed by immunoelectrophoresis and specific antinodies obtained in rabbits are presented. The variants are characterized by differences in electrophoretic mobility: one being fast, one slow, and one of an intermediate rate. To find out possible differences with respect to the effect of the trypsin and chymotrypsin on the three variants, they were treated with the enzymes before immunoelectrophoresis. Migration was accelerated in all cases, after complexing with the enzymes, but the differences in the relative positions of the variants were maintained. The trypsin- and chymotrypsin-binding capacities of these three forms seem to differ, as suggested by the results presented in this report.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Berne, B. H., Dray, S., Knight, K. L.: Immunological relationship of serum α-macroglobulins in the human, rat and rabbit. Proc. Soc. exp. Biol. (N.Y.) 138, 531–535 (1971)
Gallango, M. L., Castillo, O.: α2-macroglobulin polymorphism: a new genetic system detected by immunoelectrophoresis. J. Immunogenet. 1, 243–248 (1974)
Canrot, P. O.: Interaction of plasmin and trypsin with α-macroglobulin. Acta chem. scand. 21, 602–608 (1967)
Got, R., Monray, H., Moretti, J.: Etude biochimique de α1-macroglobuline du serum de lapin 1. Préparation et propriétés physicochimiques. Biochim. biophys. Acta (Amst.) 107, 278–285 (1965)
Harpel, P. C.: Studies on human plasma α2-macroglobulin-enzyme interactions. J. exp. Med. 138, 508–521 (1973)
Haverback, B. J., Dyce, B., Bundy, H. F., Wirtschafter, S. K., Edmonson, H. A.: Protein binding of pancreatic proteolytic enzymes. J. clin. Invest. 41, 972–980 (1962)
James, K., Taylor, F. B., Jr., Fudenberg, H. H.: Trypsin stabilizers in human serum. The role of α2-macroglobulin. Clin. chim. Acta 13, 359–368 (1966)
Lanchantin, G. F., Plesset, M. L., Friedmann, J. A., Hart, D. W.: Dissociation of esterolytic and clotting activities of thrombin by trypsin-binding macroglobulin. Proc. Soc. exp. Biol. (N.Y.) 121, 444–449 (1966)
Morelis, P., Bickhart, J. M., Arnous, J. M., Fontanzes, R.: Particularités de l'apparition des anticorps et de la production d'α-macroglobuline chez les souris thymectomisées a la naissance. C.R. Soc. Biol. (Paris) 164, 846–852 (1970)
Ohlsson, K.: Isolation and partial characterization of two related trypsin binding α-macroglobulins of dog plasma. Biochim biophys. Acta (Amst.) 236, 84–91 (1971)
Saunders, R., Dyce, B. J., Vannier, W. E., Haverback, B. J.: The separation of alpha-2-macroglobulin into five components with differing electrophoretic and enzyme binding properties. J. clin. Invest. 50, 2376–2383 (1971)
Scheidegger, J. J.: Une microméthode de l'immuno-électrophorése. Int. Arch. Allergy 7, 103–110 (1955)
Schultze, H. E., Göllner, I., Heide, K., Shönnenberger, M., Schwick, G.: Zur Kenntnis der Globuline des menschlichen Normalserums. Z. Naturforsch. 108, 463–473 (1955)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Gallango, M.L., Castillo, O. Variants of normal human α2-macroglobulin. immunoelectrophoresis and enzyme-binding effect. Hum Genet 26, 71–74 (1975). https://doi.org/10.1007/BF00280286
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00280286