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The chemical determination of section thickness

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Summary

In quantitative histochemistry it is frequently desirable to know the exact thickness of the sections. The nucleic acid content of the tissue has been used as the basis of a method for determining section thickness. Such determinations agree remarkably well with the setting on the cryostat microtome although considerable variations in thickness can occur in sections cut at different speeds.

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Authors and Affiliations

  1. Division of Cellular Biology, The Mathilda and Terence Kennedy Institute of Rheumatology, Bute Gardens, Hammersmith, London

    R. G. Butcher

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  1. R. G. Butcher
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Butcher, R.G. The chemical determination of section thickness. Histochemie 28, 131–136 (1971). https://doi.org/10.1007/BF00279857

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  • DOI: https://doi.org/10.1007/BF00279857

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