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Shared control of maltose induction and catabolite repression of the MAL structural genes in Saccharomyces

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Abstract

Maltose utilization in yeast requires the presence of any one of the five unlinked, homologous MAL loci. Transcription of the two structural genes MALT (permease) and MALS (maltase) is induced by maltose and catabolite-repressed by glucose. MAL6T and MAL6S share a common 5′ intergenic sequence; deletion studies within this sequence revealed a bi-directionally functioning upstream activation sequence (UASM) consisting of four 11bp homologous sites. Activation of these sites by the MALR protein results in the coordinate expression of MAL6T and MAL6S. The basal promoter activates MALS expression to a greater extent than MALT and is located in a region that overlaps UASM. Deletion of several subsites within the UASM has an asymmetric effect on MAL gene expression, having a greater affect on MALT than on MALS. Catabolite repression of MAL6T and MAL6S by glucose is controlled at several levels. Using disruption mutants, the positively acting MAL1R protein was also found to play a role in catabolite repression of MAL6T and MAL6S.

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Author notes

  1. Bei Yao

    Present address: Department of Medicine, Sloan Kettering Institute, Signal Transduction Laboratory, RRL 409/COST 6017, 1275 York Avenue, 10021, New York, NY, USA

Authors and Affiliations

  1. Department of Biochemistry, Albert Einstein College of Medicine, 1300 Morris Park Ave., 10461, Bronx, NY, USA

    Bei Yao, Paul Sollitti, Xiaolong Zhang & Julius Marmur

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  1. Bei Yao
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  2. Paul Sollitti
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  3. Xiaolong Zhang
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  4. Julius Marmur
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Communicated by C.P. Hollenberg

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Yao, B., Sollitti, P., Zhang, X. et al. Shared control of maltose induction and catabolite repression of the MAL structural genes in Saccharomyces . Molec. Gen. Genet. 243, 622–630 (1994). https://doi.org/10.1007/BF00279571

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  • DOI: https://doi.org/10.1007/BF00279571

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