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Characterization of the MKS1 gene, a new negative regulator of the Ras-cyclic AMP pathway in Saccharomyces cerevislae

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Abstract

In order to isolate genes that function downstream of the Ras-cAMP pathway in Saccharomyces cerevisiae, a YEp13-based genomic library was screened for clones that inhibit growth of cells with diminished A-kinase activity. One such gene, MKS1, was found to encode a hydrophilic 52 kDa protein that shares weak homology with the yeast SPT2/SIN1 gene product. Three lines of evidence suggest that the MKS1 gene product is a negative regulator downstream of the Ras-cAMP pathway: (i) overexpression of MKS1 inhibits growth of cyrl disruptant cells on YPD medium containing a low concentration of cAMP; (ii) overexpression of MKS1 does not affect TPK1 expression; and (iii) the temperature-sensitive cyrl-230 mutation is partially suppressed by mks1 disruption. The mks1 mutant shows similar phenotypes to gal11/spt13, i.e., it cannot grow on YPGal containing ethidium bromide at 25°C, or on YPGly or SGal at 37°C. The mks1 gal11 double mutant shows more marked phenotypic changes than the single mutants. These results suggest that MKS1 is involved in transcriptional regulation of several genes by cAMP.

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  1. Department of Biology, Faculty of Science, University of Tokyo, Hongo, 113, Bunkyo-ku, Tokyo, Japan

    Akira Matsuura & Yasuhiro Anraku

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  1. Akira Matsuura
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  2. Yasuhiro Anraku
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Communicated by C. Hollenberg

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Matsuura, A., Anraku, Y. Characterization of the MKS1 gene, a new negative regulator of the Ras-cyclic AMP pathway in Saccharomyces cerevislae . Molec. Gen. Genet. 238, 6–16 (1993). https://doi.org/10.1007/BF00279524

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  • DOI: https://doi.org/10.1007/BF00279524

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