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Characterization of the cyrl-2 UGA mutation in Saccharomyces cerevisiae

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Summary

cyrl-2 is a temperature-sensitive mutation of the yeast adenylate cyclase structural gene, CYR1. The cyrl-2 mutation has been suggested to be a UGA mutation since a UGA suppressor SUP201 has been isolated as a suppressor of the cyrl-2 mutation. Construction of chimeric genes restricted the region containing the cyrl-2 mutation, and the cyrl-2 UGA mutation was identified at codon 1282, which lies upstream of the region coding for the catalytic domain of adenylate cyclase. Alterations in the region upstream of the cyrl-2 mutation site result in null mutations. The complete open reading frame of the cyrl-2 gene expressed under the control of the GAL1 promoter complemented cyrl-dl in a galactose-dependent manner. These results suggest that at the permissive temperature weak readthrough occurs at the cyrl-2 mutation site to produce low levels of active adenylate cyclase. An endogenous suppressor in yeast cells is assumed to be responsible for this readthrough.

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Author notes

  1. Takashi Morishita & Akira Matsuura

    Present address: Department of Biology, Faculty of Science, University of Tokyo, 113, Bunkyo-ku, Tokyo, Japan

Authors and Affiliations

  1. Institute of Applied Microbiology, University of Tokyo, 113, Bunkyo-ku, Tokyo, Japan

    Takashi Morishita & Akira Matsuura

  2. R and D Laboratories 1, Nippon Steel Corporation, 211, Kawasaki, Japan

    Isao Uno

Authors
  1. Takashi Morishita
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  2. Akira Matsuura
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  3. Isao Uno
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Communicated by C.P. Hollenberg

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Morishita, T., Matsuura, A. & Uno, I. Characterization of the cyrl-2 UGA mutation in Saccharomyces cerevisiae . Molec. Gen. Genet. 237, 463–466 (1993). https://doi.org/10.1007/BF00279452

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  • DOI: https://doi.org/10.1007/BF00279452

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