Skip to main content
SpringerLink
Log in

The two apolipoprotein loci apoA-I and apoA-IV are closely linked in man

  • Original Investigations
  • Published:
Human Genetics Aims and scope Submit manuscript

Summary

In man the closely linked genes for the apolipoproteins A-I and C-III have been assigned to chromosome 11. Linkage studies performed in a Norwegian family with a mutant apoA-I gene established a close linkage between the loci for apoA-I and apoA-IV. For both sexes combined, the peak lod score was 3.01 at a recombination fraction of θ=0.00. Thus this study adds the locus of apoA-IV to the previously reported apolipoprotein gene cluster on chromosome 11. The previously unidentified polymorphic serum protein, USP1, is by immunochemical and electrophoretical methods identified as apoA-IV. ApoA-IV typing should be a valuable tool in elucidating the genomic organization of chromosome 11.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Anderson L, Anderson NG (1977) High resolution two-dimensional electrophoresis of human plasma proteins. Proc Natl Acad Sci USA 12:5421–5425

    Google Scholar 

  • Anderson NG, Anderson NL (1978a) Analytic techniques for cell fractions. XXI. Two-dimensional analysis of serum and tissue proteins: multiple isoelectric focusing. Anal Biochem 85:331–340

    Google Scholar 

  • Anderson NL, Anderson NG (1978b) Analytic techniques for cell fractions. XXII. Two-dimensional analysis of serum and tissue proteins: multiple gradient-slab gel electrophoresis. Anal Biochem 85:341–354

    Google Scholar 

  • Baker WC, Dayhoff MO (1977) Evolution of lipoproteins deduced from protein sequence data. Comp Biochem Physiol 57 [B]:309–315

    Google Scholar 

  • Beisiegel U, Utermann G (1979) An apolipoprotein homolog of rat apolipoprotein A-IV in human plasma. Eur J Biochem 93:601–608

    Google Scholar 

  • Brewer HB Jr, Fairwell T, La Rue A, Ronan R, Houser A, Bronzert TJ (1978) The amino acid sequence of human apoA-I, an apolipo-protein isolated from high density lipoproteins. Biochem Biophys Res Commun 80:623–630

    Google Scholar 

  • Fielding CJ, Shore VG, Fielding PE (1972) A protein cofactor of lecithin: cholesterol acyltransferase. Biochem Biophys Res Commun 46:1493–1498

    Google Scholar 

  • Franceschini G, Sirtori CR, Capurso A, Weisgraber KH, Mahley RW (1980) A-I-Milano apoprotein. Decreased high density lipoprotein cholesterol levels with significant lipoprotein modifications and without clinical atherosclerosis in an Intalian family. J Clin Invest 66:892–900

    Google Scholar 

  • Gerald PS, Grzeschik KH (1984) Report of the committee on the genetic constitution of chromosome 10, 11 and 12. Human Gene Mapping 7, Los Angeles Conference 1983. Cytogenet Cell Genet 37:103–126

    Google Scholar 

  • Heiss G, Johnson NJ, Reiland S, Davis CE, Tyroler HA (1980) The epidemiology of plasma high-density lipoprotein cholesterol levels. The Lipid Research Clinics Program Prevalence Study. Circulation 62 [Suppl IV]:116–136

    Google Scholar 

  • Karathanasis SK, Norum RA, Zannis VI, Breslow IL (1983a) An inherited polymorphism in the human apolipoprotein A-I gene locus related to the development of atherosclerosis. Nature 301:718–702

    Google Scholar 

  • Karathanasis SK, McPherson J, Zannis VI, Breslow JL (1983b) Linkage of human apolipoproteins A-I and C-III genes. Nature 304:876–878

    Google Scholar 

  • Kay LL, Ronan R, Schaefer EJ, Brewer HB Jr (1982) Tangier disease: a structural defect in apolipoprotein A-I (apoA-I-Tangier). Proc Natl Acad Sci USA 79:2485–2489

    Google Scholar 

  • Law SW, Gray G, Brewer HB Jr, Naylor SL, Sakaguchi AY (1984) Human apo A-I gene resides in the p11→q13 region of chromosome 11. Human Gene Mapping 7, Los Angeles Conference 1983. Cytogenet Cell Genet 37:520

    Google Scholar 

  • Menzel HJ, Kladetzky RG, Assmann G (1982a) One-step screening method for the polymorphism of apolipoproteins A-I, A-II, A-IV. J Lipid Res 23:915–922

    Google Scholar 

  • Menzel HJ, Kovary PM, Assmann G (1982b) Apolipoprotein A-IV polymorphism in man. Hum Genet 62:349–352

    Google Scholar 

  • Menzel HJ, Assmann G, Rall SC, Weisgraber KH, Mahley RW (1984) Human apolipoprotein A-I polymorphism. Identification of amino acid substitutions in three electrophoretic variants of the Münster-3 type. J Biol Chem 259:3070–3076

    Google Scholar 

  • Morton NE (1955) Sequential tests for the detection of linkage. Am J Hum Genet 7:277–318

    Google Scholar 

  • Norum RA, Jeffrey BL, Goldstein S, Angel A, Goldberg RB, Block WD, Noffze DK, Dolphin PJ, Edelglass J, Bogorad DD, Alaupovic P (1982) Familial deficiency of apolipoproteins A-I and C-III and precocious coronary-artery disease. N Engl J Med 306:1513–1519

    Google Scholar 

  • O'Farrell PH (1975) High resolution two-dimensional electrophoresis of proteins. J Biol Chem 250:4007–4021

    Google Scholar 

  • Olaisen B, Mevåg B, Teisberg P, Gedde-Dahl T Jr, Helland R, Siverts A, Jonassen R, Hjalmarsson K, Swan T (1981) Plasma protein polymorphisms in two-dimensional electrofocusing/SDS electrophoresis. In: Proceedings from the 9th International Congress of the Society for Forensic Haemogenetics, Bern, 1981, pp 543–555

  • Olaisen B, Gedde-Dahl T Jr, Teisberg P (1982) Linkage studies in three “new” plasma polypeptide loci. Abstract. Cytogenet Cell Genet 32:305

    Google Scholar 

  • Schamaun O, Olaisen B, Gedde-Dahl T Jr, Teisberg P (1983a) Genetic studies of an apoA-I lipoprotein variant. Hum Genet 64: 380–383

    Google Scholar 

  • Schamaun O, Olaisen B, Mevåg B, Gedde-Dahl T Jr, Teisberg P (1984b) Linkage relations of human apoA-I. A possible linkage to apoA-IV. Human Genet Mapping 7, Los Angeles Conference 1983. Cytogenet Cell Genet 37:577

    Google Scholar 

  • Tracy RP, Currie RM, Young DS (1982) Two-dimensional gel electrophoresis of serum specimens from a normal population. Clin Chem 28:890–899

    Google Scholar 

  • Utermann G, Beisiegel U (1979) Apolipoprotein A-IV: a protein occurring in human mesenteric lymph chylomicrons and free in plasma. Eur J Biochem 99:333–343

    Google Scholar 

  • Utermann G, Feussner G, Franceschini G, Haas J, Steinmetz A (1982) Genetic variants of group A apolipoproteins. Rapid method for screening and characterization without ultracentrifugation. J Biol Chem 257:501–507

    Google Scholar 

  • Weinberg RB, Scanu AM (1983) Isolation and characterization of human apolipoprotein A-IV from lipoprotein-depleted serum. J Lipid Res 24:52–59

    Google Scholar 

  • Weisgraber KH, Bersot TP, Mahley RW (1978) Isolation and characterization of an apoprotein from the d<1.006 lipoproteins of human and canine lymph homologous with the rat A-IV apoprotein. Biochem Biophys Res Commun 85:287–292

    Google Scholar 

  • Wøien D (1970) Description of MOSM Program for genetic linkage analysis. Norwegian Computing Center, Oslo, Norway

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Medical Department, Rikshospitalet, Oslo 1, Norway

    O. Schamaun & P. Teisberg

  2. Institute of Forensic Medicine, University of Oslo, Rikshospitalet, Oslo 1, Norway

    B. Olaisen & B. Mevåg

  3. Department of Genetics, NHIK, Radiumhospitalet, Oslo 3, Norway

    T. Gedde-Dahl Jr.

  4. Central Public Health Laboratory, Helsinki, Finland

    C. Ehnholm

Authors
  1. O. Schamaun
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. B. Olaisen
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. B. Mevåg
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. T. Gedde-Dahl Jr.
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. C. Ehnholm
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. P. Teisberg
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Schamaun, O., Olaisen, B., Mevåg, B. et al. The two apolipoprotein loci apoA-I and apoA-IV are closely linked in man. Hum Genet 68, 181–184 (1984). https://doi.org/10.1007/BF00279311

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00279311

Keywords

Search

Navigation