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The polymorphism of desialyzed α2HS-glycoptein (AHS): isoelectric focusing in 2.5 M urea as a method for identification of genetic variants

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Summary

Desialyzed plasma specimens were phenotyped for α2HS-glycoprotein (AHS) using polyacrylamide isoelectric focusing (IEF) in the range pH 5–6 in 2.5 M urea, followed by immunoblotting. The technique used in this study is easy to perform and can differentiate the gene products of all the currently known variants of α2HS-glycoprotein except for AHS 4.

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Authors and Affiliations

  1. Department of Forensic Medicine, Yamagata University School of Medicine, 990-23, Yamagata, Japan

    K. Umetsu & T. Suzuki

  2. Department of Legal Medicine, Tottori University School of Medicine, 683, Yonago, Japan

    I. Yuasa

Authors
  1. K. Umetsu
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  2. I. Yuasa
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  3. T. Suzuki
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Umetsu, K., Yuasa, I. & Suzuki, T. The polymorphism of desialyzed α2HS-glycoptein (AHS): isoelectric focusing in 2.5 M urea as a method for identification of genetic variants. Hum Genet 73, 372–373 (1986). https://doi.org/10.1007/BF00279104

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  • DOI: https://doi.org/10.1007/BF00279104

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