Summary
Two probes from the random human cosmid c1-37 detect restriction fragment length polymorphisms in humans. The loci revealed by these probes are in linkage equilibrium and constitute a compound polymorphic locus with a polymorphism information content of 0.54. A somatic cell hybrid panel has been used to map the probes to chromosome 20; in situ hybridization studies confirm this localization and indicate that the locus is on 20q13. This is the first polymorphic locus to be assigned to the long arm of chromosome 20.
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Bhattacharya SS, Wright AF, Clayton JF, Price WH, Phillips CI, McKeown CME, Jay M, Bird AC, Pearson PL, Southern EM, Evans HJ (1984) Close genetic linkage between X-linked retinitis pigmentosa and a restriction fragment length polymorphism identified by recombinant DNA probe L1.28. Nature 309:253–255
Botstein D, White RL, Skolnick M, Davis RW (1980) Construction of a genetic linkage map in man using restriction fragment length polymorphisms. Am J Hum Genet 32:314–331
Bufton L, Bruns GA, Magenis RE, Tomar D, Shaw D, Brook D, Litt M (1986) Four restriction fragment length polymorphisms revealed by probes from a single cosmid map to chromosome 19. Am J Hum Genet 38:447–460
Buroker NE, Magenis RE, Weliky K, Bruns G, Litt M (1986) Four restriction fragment length polymorphisms revealed by probes from a single cosmid map to human chromosome 12q. Hum Genet 72:86–94
Cavenee WK, Dryja TP, Phillips RA, Benedict WF, Godbout R, Gallie BR, Murphree AL, Strong LC, White RL (1983) Expression of recessive alleles by chromosomal mechanisms in retinoblastoma. Nature 305:779–784
Gusella JF, Wexler NS, Conneally PM, Naylor SL, Anderson MA, Tanzi RE, Watkins PC, Ottina K, Wallace MR, Sakaguchi AY, Young AB, Shoulson I, Bonilla E, Martin JB (1983) A polymorphic DNA marker genetically linked to Huntington's disease. Nature 306:234–238
Hartl DL (1980) Principles of population genetics. Sinauer, Sunderland, Mass, pp 110–111
Koufos A, Hansen MF, Lampkin BC, Workman ML, Copeland NG, Jenkins NA, Cavenee WK (1984) Loss of alleles at loci on human chromosome 11 during genesis of Wilms' tumor. Nature 309:170–172
Litt M, White RL (1985) A highly polymorphic locus in human DNA revealed by cosmid-derived probes. Proc Natl Acad Sci USA 82: 6206–6210
Litt M, Bruns GA, Sheehy R, Magenis RE (1986) A highly polymorphic locus in human DNA revealed by probes from cosmid 1–5 maps to chromosome 2q35→37. Am J Hum Genet
Mayo KE, Cerelli GM, Lebo RV, Bruce BD, Rosenfeld MG, Evans RM (1985) Gene encoding human growth-hormone-releasing factor precursor: structure, sequence, and chromosomal assignment. Proc Natl Acad Sci USA 82:63–67
Murray JM, Davies KE, Harper PS, Meredith L, Mueller CR, Williamson R (1982) Linkage relationship of a cloned DNA sequence on the short arm of the X chromosome to Duchenne muscular dystrophy. Nature 300:69–71
Reeders ST, Breuning MH, Davies KE, Nicholls RD, Jarman AP, Higgs DR, Pearson PL, Weatherall DJ (1985) A highly polymorphic DNA marker linked to adult polycystic kidney disease on chromosome 16. Nature 317:542–544
Riddell DC, Mallonee R, Phillips JA, Parks JS, Sexton LA, Hamerton JL (1985) Chromosomal assignment of human sequences encoding arginine vasopressin-neurophysin 11 and growth hormone ealeasing factor. Somatic Cell Mol Genet 11:189–195
Sealey PG, Whittaker PA, Southern EM (1985) Removal of repeated sequences from hybridization probes. Nucleic Acids Res 13:1905–1922
Tischfield JA, Creagan RP, Nichols EA, Ruddle FH (1974) Assignment of a gene for adenosine deaminase to human chromosome 20. Hum Hered 24:1–11
Tsui LC, Buchwald M, Barker D, Braman JC, Knowlton R, Schumm JW, Eiberg H, Mohr J, Kennedy D, Plasvic N, Zsiga M, Markiewicz D, Akots G, Brown V, Helms C, Gravius T, Parker C, Rediker K, Donis-Keller H (1985) Cystic fibrosis locus defined by a genetically linked polymorphic DNA marker. Science 230: 1054–1056
Willard HF, Skolnick MH, Pearson PL, Mandel J-L (1985) Report of the Committee on Human Gene Mapping by Recombinant DNA Techniques. 8th International Workshop on Human Gene Mapping. Cytogenet Cell Genet 40:360–489
White R, Leppert M, Bishop DT, Barker D, Berkowitz J, Brown C, Callahan P, Holm T, Jerominski L (1985a) Construction of linkage maps with DNA markers for human chromosomes. Nature 313:101–105
White R, Woodward S, Leppert M, O'Connell P, Hoff M, Herbst J, Lalouel J-M, Dean M, Vande Woude G (1985b) A closely linked genetic marker for cystic fibrosis. Nature 313:382–384
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Litt, M., Sheehy, R., Bruns, G.A. et al. A polymorphic locus on the long arm of chromosome 20 defined by two probes from a single cosmid. Hum Genet 73, 340–345 (1986). https://doi.org/10.1007/BF00279097
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DOI: https://doi.org/10.1007/BF00279097