Summary
Sperm and lymphocyte chromosome studies in a normal, fertile male have shown a high degree of coincidence between chromosome lesions and fragile sites in both types of cells. In this donor we also found that some fragile sites expressed in sperm chromosomes coincided with those expressed in lymphocyte chromosomes. These results indicate that the chromosome lesions expressed in sperm do not occur at random and that they are not technical artifacts. The fragility expression in sperm chromosomes could reflect in vivo conditions. The presence in some sperm metaphases of acentric fragments suggests that chromosome fragility can result in the loss of chromosome fragments or give rise to de novo structural rearrangements. However, the incidence of sperm with chromosomal abnormalities observed in this man was within the normal range.
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Barbi G, Steinbach P, Vogel W (1984) Nonrandom distribution of methotrexate-induced aberrations on human chromosomes. Detection of further folic acid sensitive fragile site. Hum Genet 68:290–294
Benet J, Genescà A, Navarro J, Egozcue J, Templado C (1986) G-banding of human sperm chromosomes. Hum Genet 73:181–182
Brandriff B, Gordon L, Ashwoth L, Watchmaker G, Carrano AV, Wyrobek A (1984) Chromosomal abnormalities in human sperm. Comparisons among four healthy men. Hum Genet 66:193–201
Brandriff B, Gordon L, Watchmaker G (1985a) Human sperm chromosomes obtained from hamster eggs after sperm capacitation in TEST-Yolk buffer. Gamete Res 11:253–259
Brandriff B, Gordon L, Ashworth L, Watchmaker G, Moore DH II, Wyrobek AJ, Carrano AV (1985b) Chromosomes of human sperm: variability among normal individuals. Hum Genet 70:18–24
Chernos JE, Rattner JB, Martin RH (1986) An investigation of human sperm pronuclear chromosome “gaps” using scanning electron microscopy. Cytogenet Cell Genet 42:57–61
Craig-Holmes AP, Strong LC, Goodacre A, Pathak S (1987) Variation in the expression of aphidicolin-induced fragile sites in human lymphocyte cultures. Hum Genet 76:134–137
Daniel A (1986) Clinical implications and classification of the constitutive fragile sites. Am J Med Genet 23:419–427
Fuster C, Miró R, Templado C, Egozcue J (1986) Inter-individual variability of fragile site expression in normal individuals. 7th International Congress of Human Genetics, Berlin 1986, pp 213 (abstr)
Garcia-Sagredo JM, San Román C, Gallego-Gomez ME, Lledo G (1984) Fragile chromosome 16(q22) causes a balanced translocation at the same point. Hum Genet 65:211–213
Guichaoua A, Mattei MG, Mattei JF, Giraud F (1982) Aspects genetiques des sites fragiles autosomiques. A propos de 40 cas. J Genet Hum 30:183–197
Hecht F (1986) Rare, polymorphic, and common fragile sites: a classification. Hum Genet 74:207–208
ISCN (1985) An international system for human cytogenetic nomenclature. Harnden DG, Klinger HP (eds) Published in collaboration with Cytogenet Cell Genet. Karger, Basel
Marlhens F, Al Achkar W, Aurias A, Couturier J, Dutrillaux AM, Gerbault-Sereau M, Hoffschir F, Lamoliatte E, Lefrançois D, Lombard M, Muleris M, Prieur M, Prod'homme M, Sabatier L, Viegas-Péquignot E, Volobouer V, Dutrillaux B (1986) The rate of chromosome breakage is age dependent in lymphocytes of adult controls. Hum Genet 73:290–297
Martin RH (1983) A detailed method for obtaining preparations of human sperm chromosomes. Cytogenet Cell Genet 35:252–256
Martin RH (1986) A fragile site 10q25 in human sperm chromosomes. J Med Genet 23:279
Martin RH, Balkan W, Burns K, Rademaker AW, Lin CC, Rudd NL (1983) The chromosome constitution of 1000 human spermatozoa. Hum Genet 63:305–309
Martin RH, Rademaker AW, Hildebrand K, Long-Simpson L, Peterson D, Yamamoto J (1987) Variation in the frequency and type of sperm chromosomal abnormalities among normal men. Hum Genet 77:108–114
Navarro J, Benet J, Genescà A, Castell N, Egozcue J, Templado C (1987) Study of human sperm chromosomes by sequential transmission and scanning electron microscopy. Hum Reprod 2:583–587
Shabtai F, Hart J, Klar D, Halbrecht I, (1986) Familial fragile sites found at the cancer breakpoint (1)(q32). Hum Genet 73:232–234
Sutherland GR (1979) Heritable fragile sites on human chromosomes. I. Factors affecting expression in lymphocyte culture. Am J Hum Genet 31:125–135
Sutherland GR, Mattei JF (1987) Report of the committee on cytogenetic markers. (9th International Workshop on Human Gene Mapping). Cytogenet Cell Genet 46:316–324
Templado C, Benet J, Genescà A, Navarro J, Caballin MR, Miró R, Egozcue J (1988) Human sperm chromosomes. Hum Reprod 3:133–138
Tommerup N, Nielsen J, Mikkelsen M (1985) A folate sensitive heritable fragile site at 19p13. Clin Genet 27:510–514
Voullaire LE, Webb GC, Leversha MA (1987) Chromosome deletion at 11q23 in an abnormal child from a family with inherited fragility at 11q23. Hum Genet 76:202–204
Yunis JJ, Soreng AL (1984) Constitutive fragile sites and cancer. Science 226:1199–1204
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Benet, J., Fuster, C., Genescà, A. et al. Expression of fragile sites in human sperm and lymphocyte chromosomes. Hum Genet 81, 239–242 (1989). https://doi.org/10.1007/BF00278996
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DOI: https://doi.org/10.1007/BF00278996