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Isolation and characterization of the complete human β-myosin heavy chain gene

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Summary

The entire gene coding for the human β-myosin heavy chain has been isolated from genomic EMBL3A phage libraries by chromosomal walking starting from clone gMHC-1, reported earlier (Appelhans and Vosberg 1983). gMHC-1 has been shown to carry coding information for the C-terminal two-thirds of β-myosin heavy chain, which is expressed in cardiac muscle and in slow skeletal muscle fibers (Lichter et al. 1986). Three DNA clones were identified as overlapping with gMHC-1 by restriction mapping and DNA sequencing. They span a 30-kb region in the genome. About 22 kb extend from the initiation codon ATG to the poly(A) addition site. The clones include about 4 kb of 5′ flanking sequences upstream of the promoter. Comparisons of β- and α-myosin heavy chain sequences indicate that gene duplication of the cardiac myosin heavy chain isogenes preceded the mammalian species differentiation.

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Author notes

  1. Peter Lichter

    Present address: Department of Human Genetics, Yale University School of Medicine, 06510, New Haven, CT, USA

Authors and Affiliations

  1. Abteilung für Molekulare Biologie, Max-Planck-Institut für medizinische Forschung, Jahnstrasse 29, D-6900, Heidelberg, Germany

    Klaus W. Diederich, Ingrid Eisele, Thomas Ried, Thomas Jaenicke, Peter Lichter & Hans-Peter Vosberg

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  1. Klaus W. Diederich
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  2. Ingrid Eisele
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  3. Thomas Ried
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  4. Thomas Jaenicke
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  5. Peter Lichter
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  6. Hans-Peter Vosberg
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Diederich, K.W., Eisele, I., Ried, T. et al. Isolation and characterization of the complete human β-myosin heavy chain gene. Hum Genet 81, 214–220 (1989). https://doi.org/10.1007/BF00278991

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  • DOI: https://doi.org/10.1007/BF00278991

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