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Effects of various cyclophosphamide concentrations in vivo on sister chromatid exchanges (SCE) and chromosome aberrations of Chinese hamster bone marrow cells

Comparison of Two Methods Measuring the Mutagenicity of a Test Compound

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Summary

The in vivo SCE formation and the induction of chromosome aberrations in the bone marrow of Chinese hamsters (Cricetulus griseus) were studied after various concentrations of cyclophosphamide, and the sensitivity of the two test methods was compared. The administration of 1.0, 5.0, 13.3, 25.0, and 40.0 mg/kg body weight induced a dose-dependent increase in SCE. The frequency of chromosome aberration, however, was not increased significantly with doses of 1.0 and 5.0 mg/kg body weight. Only with doses of more than 13.3 mg is a significant induction of chromosome aberrations seen. Therefore the SCE test system seems to be 10 times more sensitive than the induction of chromosome aberrations in the same cell type.

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Authors and Affiliations

  1. Institut für Humangenetik und Anthropologie der Universität Düsseldorf, Universitätsstraße 1, Gebäude 23.12, D-4000, Düsseldorf 1, Federal Republic of Germany

    G. Roszinsky-Köcher & G. Röhrborn

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  1. G. Roszinsky-Köcher
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  2. G. Röhrborn
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This work is a part of the M.D. thesis of G. Roszinsky-Köcher, to whom offprint requests should be sent

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Roszinsky-Köcher, G., Röhrborn, G. Effects of various cyclophosphamide concentrations in vivo on sister chromatid exchanges (SCE) and chromosome aberrations of Chinese hamster bone marrow cells. Hum Genet 46, 51–55 (1979). https://doi.org/10.1007/BF00278901

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  • DOI: https://doi.org/10.1007/BF00278901

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