Skip to main content
Log in

Sister chromatid exchange in immature haemopoietic cells, T-and B-lymphocytes

  • Short Communications
  • Published:
Human Genetics Aims and scope Submit manuscript

Summary

T-lymphocytes, B-lymphocytes, and cells derived from human haemopoietic progenitor cells were analysed for their sister chromatid exchange (SCE) rate. T-and B-lymphocytes were separated and stimulated with phytohaemagglutinin (PHA) and Epstein Barr (EB) virus respectively, while the progenitor cells were cultured in the mixed colony assay. B-lymphocytes had a significantly lower SCE rate than either the T-lymphocytes or cells derived from progenitor cells.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

References

  • Becher R, Sandberg AA (1984) Sister chromatid exchange levels and cell cycle time in human bone marrow cells and lymphocytes. Cancer Genet Cytogenet 11:19–23

    Google Scholar 

  • Becher R, Schmidt CG, Theis G, Hossfeld DK (1979) The rate of sister chromatid exchange in normal human bone marrow cells. Hum Genet 50:213–216

    Google Scholar 

  • Block AM, Shiraishi Y, Sandberg AA (1982) SCE in Bloom syndrome B and T lymphocytes. Clin Genet 21:184–186

    Google Scholar 

  • Bodger MP, McGiven AR, Fitzgerald PH (1979) Mitogenic proteins of pokeweed. Immunology 37:785–792

    Google Scholar 

  • Bodger MP, Izaguirre CA, Blacklock HA, Hoffbrand AV (1983) Surface antigenic determinants on human pluripotent and unipotent haemopoietic progenitor cells. Blood 61:1006–1010

    Google Scholar 

  • Brown RL, Crossen PE (1976) Increased incidence of sister chromatid exchanges in Rauscher leukaemia virus infected mouse embryo fibroblasts. Exp Cell Res 103:418–420

    Google Scholar 

  • Carson DA, Kaye J, Matsumoto S, Seegmiller JE, Thompson L (1979) Biochemical basis for the enhanced toxicity of deoxyribonucleosides toward malignant human T cell lines. Proc Natl Acad Sci USA 76:2430–2433

    Google Scholar 

  • Crossen PE (1983) Arsenic and SCE in human lymphocytes. Mutat Res 119:415–419

    Google Scholar 

  • Crossen PE, Broderick RD, Durie BGM, Trent JM (1985) Proliferative characteristics and sister chromatid exchange (SCE) of colony forming cells (CFU-S) in acute myelogenous leukemia. Cancer Genet Cytogenet 14:267–273

    Google Scholar 

  • Fauser AA, Messner HA (1978) Granuloerythropoietic colonies in human bone marrow, peripheral blood and cord blood. Blood 52:1243–1248

    Google Scholar 

  • Fauser AA, Kanz L, Bross KJ, Lohr GW (1983) Identification of pre-B cells in multilineage haemopoietic colonies (CFU-GEMMT). Blood 62:135 [Suppl 1]

    Google Scholar 

  • Knuutila S, Helminen E, Vuopio P, de la Chapelle A (1978) Sister chromatid exchanges in human bone marrow cells. Hereditas 88:189–196

    Google Scholar 

  • Lindblad A, Lambert B (1981) Relation between sister chromatid exchange, cell proliferation and proportion of B and T cells in human lymphocyte cultures. Hum Genet 57:31–34

    Google Scholar 

  • Messner HA, Izaguirre CA, Jamal N (1981) Identification of T lymphocytes in human mixed hemopoietic colonies. Blood 58:402–405

    Google Scholar 

  • Morgan WF, Crossen PE (1977) The incidence of sister chromatid exchanges in cultured human lymphocytes. Mutat Res 42:305–312

    Google Scholar 

  • Nichols WW, Bradt CI, Toji LH, Godley M, Segawa M (1978) Induction of sister chromatid exchanges by transformation with simian virus 40. Cancer Res 38:960–964

    Google Scholar 

  • Santesson B, Lindahl-Kiessling K, Mattsson A (1979) SCE in B and T lymphocytes. Possible implications for Bloom's syndrome. Clin Genet 16:133–135

    Google Scholar 

  • Shiraishi Y, Weinfeld H, Minowada J, Sandberg AA (1976) Dependency of sister chromatid exchange in T-and B-cells on the incorporation of deoxyribonucleosides into chromosomal DNA. JNCI 57:1217–1221

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Crossen, P.E., Godwin, J.M. & Bodger, M.P. Sister chromatid exchange in immature haemopoietic cells, T-and B-lymphocytes. Hum Genet 72, 101–103 (1986). https://doi.org/10.1007/BF00278828

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00278828

Keywords

Navigation