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Regional assignment of human liver-type 6-phosphofructokinase to chromosome 21q22.3 by using somatic cell hybrids and a monoclonal anti-L antibody

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Summary

The three structural loci encoding human phosphofructokinase, a key regulatory enzyme of glycolysis, are located on separate chromosomes. The gene coding for the liver-type subunit PFKL has previously been assigned to chromosome 21. We have used a subunit- and human-specific monoclonal antibody to liver PFK to detect the expression of human PFKL in hamster x human hybrid cell lines. A cell line carrying an 8;21 translocation which contains all of chromosome 21 except the band 21q22.3 was negative for the expression of PFKL whereas cell lines carrying the reciprocal 8;21 translocation were positive. In addition, a cell line with a ring chromosome 21 containing a breakpoint which excluded the distal part of the q22.3 band was negative for expression of PFKL. These results indicate that human PFKL is located on chromosome 21q22.3.

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Authors and Affiliations

  1. The Eleanor Roosevelt Institute for Cancer Research, 4200 East Ninth Avenue, B-129, 80262, Denver, CO, USA

    Margaret Van Keuren, H. Drabkin, Iris Hart & D. Patterson

  2. Department of Oncology, University of Colorado Health Sciences Center, 80262, Denver, CO, USA

    H. Drabkin

  3. Department of Medicine, University of Colorado Health Sciences Center, 80262, Denver, CO, USA

    D. Patterson

  4. Department of Biochemistry, Biophysics and Genetics, University of Colorado Health Sciences Center, 80262, Denver, CO, USA

    D. Patterson

  5. Department of Basic and Clinical Research, Scripps Clinic and Research Foundation, 92037, La Jolla, CA, USA

    D. Harker & Shobhana Vora

Authors
  1. Margaret Van Keuren
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  2. H. Drabkin
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  3. Iris Hart
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  4. D. Harker
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  5. D. Patterson
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  6. Shobhana Vora
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Van Keuren, M., Drabkin, H., Hart, I. et al. Regional assignment of human liver-type 6-phosphofructokinase to chromosome 21q22.3 by using somatic cell hybrids and a monoclonal anti-L antibody. Hum Genet 74, 34–40 (1986). https://doi.org/10.1007/BF00278782

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  • DOI: https://doi.org/10.1007/BF00278782

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