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Characterization of a complex philadelphia translocation (1p-;9q+;22q-) by gene mapping

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Summary

Human-Chinese hamster somatic cell hybrids were obtained using circulating leucocytes from a chronic myeloid leukaemia (CML) patient carrying a complex Philadelphia (Ph1) translocation (1p-; 9q+; 22q-). Hybrid clones which showed segregation of the translocation chromosomes were studied. The chromosome 22 markers ACO2, ARSA, and NAGA segregated with the 1p- derivative; and the chromosome 1 markers UMPK, PGD, and ENO1 segregated with the 9q+ derivative. Hence, molecular evidence has been obtained for the translocation of the distal part of 22q to chromosome 1 and for the translocation of the distal part of 1p to chromosome 9. No conclusions could be drawn either about translocation of chromosome 9 material or about a possible difference in breakpoint in chromosome 22 when compared with six cases of 9;22 translocations similarly studied and previously reported. In addition, a more precise mapping of PGM1 was obtained, the gene being proximal to UMPK and the breakpoint in 1p32.

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Authors and Affiliations

  1. Department of Cell Biology and Genetics, Erasmus University, P.O. Box 1738, 3000 DR, Rotterdam, The Netherlands

    A. H. M. Geurts van Kessel, A. J. van Agthoven & A. Hagemeijer

  2. Laboratory of Biochemistry, BCP Jansen Institute, University of Amsterdam, Amsterdam, The Netherlands

    P. G. de Groot

Authors
  1. A. H. M. Geurts van Kessel
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  2. A. J. van Agthoven
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  3. P. G. de Groot
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  4. A. Hagemeijer
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Geurts van Kessel, A.H.M., van Agthoven, A.J., de Groot, P.G. et al. Characterization of a complex philadelphia translocation (1p-;9q+;22q-) by gene mapping. Hum Genet 58, 162–165 (1981). https://doi.org/10.1007/BF00278702

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  • DOI: https://doi.org/10.1007/BF00278702

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