Summary
The adrenal medullae of rats were studied electron microscopically from day 13.5 of gestation. Synapses with thickening of pre- and post-synaptic membranes were first evidenced on the medullary cells of 15.5-day-old fetuses. They increased gradually in number with advancing age. In the medullary cells, a few membrane-limited granules were recognized on day 13.5 of gestation, and thereafter they increased in number. The appearance of the granules was not uniform; some of the granular contents consisted of fine or coarse particles and others contained homogeneous material of varying electron-densities. The population of these granules in the cytoplasm was different from cell to cell. Thus, the discrimination of cell types (NA- and A-cells) was not possible in the prenatal period. Granular discharge of the cells was totally absent in the normal untreated fetuses. However, upon the intraperitoneal administration of insulin to the fetus on day 16.5, 18.5, or 21.5 of gestation, granular discharge by reverse pinocytosis was first evidenced in the medullary cells of the 21.5-dayold fetus.
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Abrahams, S.J., Holtzman, E.: Secretion and endocytosis in insulin-stimulated rat adrenal medulla cells. J. Cell Biol. 56, 540–558 (1973)
Benedeczky, I., Smith, A.D.: Ultrastructural studies on the adrenal medulla of golden hamster: origin and fate of secretory granules. Z. Zellforsch. 124, 367–386 (1972)
Benedeczky, I., Somogyi, P.: Ultrastructure of the adrenal medulla of normal and insulin-treated hamsters. Cell Tiss. Res. 162, 541–550 (1975)
Chéoux, G., Roffi, J.: Dosage de l'adrénaline et de la noradrénaline dans les surrénales du foetus et du nouveauné de Rat. C.R. Acad. Sci. (Paris) 260, 6702–6705 (1965)
Coupland, R.E.: Electron microscopic observations on the structure of the rat adrenal medulla. I. The ultrastructure and organization of chromaffin cells in the normal adrenal medulla. J. Anat. (Lond.) 99, 231–254 (1965)
Daikoku, S.: A method of deencephalon in the fetal rat. Okajimas Folia anat. jap. 42, 39–49 (1966)
Daikoku, S., Ikeuchi, C., Miki, S.: Electron-microscopic studies on developing intramural ganglia of the small intestine in human and rabbit fetuses. Acta anat. (Basel) 91, 429–454 (1975)
Daikoku, S., Kinutani, M., Sako, M.: Ultrastructural study on the hypothalamic-hypophysial-adrenal axis in fetal rats. Cell Tiss. Res. 168, 549–559 (1976)
Daikoku, S., Ooki, T., Takahashi, A., Sako, M.: Studies on the functional development of rat adrenal medullary cells. Tokushima J. exp. Med. 16, 153–162 (1969)
Daikoku, S., Takahashi, T., Kojimoto, H., Watanabe, Y.G.: Secretory surface phenomena in freezeetched preparations of the adenohypophysial cells and neurosecretory fibers. Cell Tiss. Res. 136, 207–214 (1973)
De Robertis, E., Vaz Ferreira, A.: Electron microscope study of the excretion of catechol-containing droplets in the adrenal medulla. Exp. Cell Res. 12, 568–574 (1957)
De Robertis, E.D.P., Sabatini, D.D.: Submicroscopic analysis of the secretory process in the adrenal medulla. Fed. Proc. 19, Suppl. 5, 70–78 (1960)
Diner, O.: L'expulsion des granules de la médullo-surrénale chez le Hamster. C.R. Acad. Sci. (Paris) 265, 616–619 (1967)
Douglas, W.W.: Stimulus-secretion coupling: the concept and clues from chromaffin and other cells. Brit. J. Pharmacol. 34, 451–474 (1968)
Douglas, W.W., Poisner, A.M.: Evidence that the secreting adrenal chromaffin cell releases catecholamines directly from ATP-rich granules. J. Physiol. (Lond.) 183, 236–248 (1966)
Elfvin, L.-G.: The development of the secretory granules in the rat adrenal medulla. J. Ultrastruct. Res. 17, 45–62 (1967)
Grynszpan-Winograd, O.: Morphological aspects of exocytosis in the adrenal medulla. Phil. Trans. B 261, 291–292 (1971)
Hervonen, A.: Development of catecholamine-storing cells in human fetal paraganglia and adrenal medulla. A histochemical and electron microscopical study. Acta physiol. scand., Suppl. 368, 7–94 (1971)
Kirshner, N., Sage, H.J., Smith, W.J.: Mechanism of secretion from the adrenal medulla. II. Release of catecholamines and storage vesicle protein in response to chemical stimulation. Molec. Pharmacol. 3, 254–265 (1967)
Marley, E., Paton, W.D.M.: The output of sympathetic amines from the cat's adrenal gland in response to splanchnic nerve activity. J. Physiol. (Lond.) 155, 1–27 (1961)
Ratzenhofer, M., Müller, O.: Ultrastructure of adrenal medulla of the prenatal rat. J. Embryol. exp. Morph. 18, 13–25 (1967)
Smith, U., Smith, D.S., Winkler, H., Ryan, J.W.: Exocytosis in the adrenal medulla demonstrated by freeze-etching. Science 179, 79–82 (1973)
Smitten, N.A.: Cytological and ultrastructural pattern of the secretory activity of adrenomedullar cells. Arch. Anat. micr. Morph. exp. 54, 145–162 (1965)
Weakley, B.S., Coupland, R.E.: An electron microscopic study on the development of chromaffin cells in the rabbit. J. Anat. (Lond.) 99, 937 (1965)
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Daikoku, S., Kinutani, M. & Sako, M. Development of the adrenal medullary cells in rats with reference to synaptogenesis. Cell Tissue Res. 179, 77–86 (1977). https://doi.org/10.1007/BF00278463
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DOI: https://doi.org/10.1007/BF00278463