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Giemsa “banding” in metaphase chromosomes after pretreatment with inactivated trypsin

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Summary

It is shown that banding patterns after pretreatment of human chromosomes with trypsin solutions and subsequent Giemsa staining are independent of the esterase and peptidase activities of the enzyme.

Zusammenfassung

Es wird gezeigt, daß die Darstellung von Bandenmustern nach Vorbehandlung menschlicher Chromosomen mit Trypsinlösungen und nachfolgender Giemsafärbung unabhängig von der Esterase- und Peptidaseaktivität des Enzyms ist.

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References

  • Dutrillaux, B., de Grouchy, J., Finaz, C., Lejeune, J.: Mise en évidence de la structure fine des chromosomes humains par digestion enzymatique (pronase en particulier). C. R. Acad. Sci. (Paris) 273, série D, 587–588 (1971)

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  • Kato, H., Yosida, T. H.: Banding patterns of Chinese hamster chromosomes revealed by new techniques. Chromosoma (Berl.) 36, 272–280 (1972)

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  • Seabright, M.: A rapid banding technique for human chromosomes. Lancet 1971 II, 971–972

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  • Sehested, J.: Unpublished results

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Authors and Affiliations

  1. Institute of Human Genetics, University of Aarhus, Aarhus, Denmark

    Jens Sehested

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  1. Jens Sehested
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Sehested, J. Giemsa “banding” in metaphase chromosomes after pretreatment with inactivated trypsin. Hum Genet 19, 321–324 (1973). https://doi.org/10.1007/BF00278410

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  • DOI: https://doi.org/10.1007/BF00278410

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