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Humangenetik

, Volume 14, Issue 3, pp 224–231 | Cite as

Horizontal polyacrylamide electrophoresis for the determination of serum protein (haptoglobin) and red cell enzyme polymorphisms

  • H. H. Hoppe
  • Windemut Hennig
  • B. Brinkmann
Original Investigations

Summary

General and special procedures for the determination of serum (haptoglobin) and red cell enzyme polymorphisms by horizontal polyacrylamide (PAA) gel electrophoresis are given. As compared to starch gel techniques certain advantages could be established:
  1. 1.

    Improved separation and reproducibility of the patterns using PAA.

     
  2. 2.

    PAA gels are less sensitive to heating and desiccation during electrophoresis.

     
  3. 3.

    PAA gels can be stored prior to use.

     
  4. 4.

    PAA is transparent and enables densitometric studies using translucent light.

     

Keywords

Starch Internal Medicine Polyacrylamide Metabolic Disease Serum Protein 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Zusammenfassung

Allgemeine und spezielle Techniken zur Bestimmung von Serum-(Haptoglobin) und Erythrocytenenzym-Polymorphismen (SEP, PGM, Ak, ADA) mit Hilfe der horizontalen Polyacrylamidgel-Elektrophorese werden beschrieben. Im Vergleich zu Stärkegel-Techniken ergeben sich bestimmte Vorteile bei Verwendung von Polyacrylamid (PAA):
  1. 1.

    Verbesserte Trennung und Reproduzierbarkeit der Protein- und Enzymmuster.

     
  2. 2.

    Geringere Empfindlichkeit der PAA-Gele gegen Erhitzung und Austrocknung während der Elektrophorese.

     
  3. 3.

    PAA-Gele können bis zum Gebrauch gelagert werden.

     
  4. 4.

    PAA-Gele sind transparent und ermöglichen dadurch densitometrische Untersuchungen in durchfallendem Licht.

     

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Copyright information

© Springer-Verlag 1972

Authors and Affiliations

  • H. H. Hoppe
    • 1
    • 2
  • Windemut Hennig
    • 1
    • 2
  • B. Brinkmann
    • 1
    • 2
  1. 1.Zentralinstitut für das BluttransfusionswesenHamburgGermany
  2. 2.Institut für GerichtsmedizinHamburgGermany

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