Skip to main content
SpringerLink
Log in

Sur les réactions histochimiques des radicaux acides forts et notemment phosphate

On the histochemical reactions of strong acid radicals with special reference to phosphate groups

  • Published:
Histochemie Aims and scope Submit manuscript

Summary

The specificity of the reactions which are presumed to be specific for acid radicals have been tested in model experiments by means of sulphatation, phosphorylation and carboxylation of structures being rich in vic-glycol groups. The non-specifity of all routine reactions for sulphate groups is proved because they are positive also for phosphate groups. Two other reactions were modified resulting in specifity for sulphate and phosphate groups, respectively.

Résumé

L'auteur a étudié la spécificité des réactions considérées spécifiques des radicaux acides sur des modèles obtenus par sulfatation, phosphatation et carboxylation des diverses structures riches en groupes vic-glycols.

L'auteur a ainsi démontré que toutes les réactions routinaires pour la visualisation des groupes sulfat sont aspécifiques, étant donné leur positivité aussi envers les radicaux phosphate.

L'auteur démontre, au contraire, la spécificité de deux réactions, respectivement pour les radicaux sulfat et phosphate, déjà connues mais oubliées, et en propose des modifications.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Bibliographie

  • Adams, C. W. M.: Neurohistochemistry. Amsterdam-London-New York: Elsevier 1965.

    Google Scholar 

  • Battaglia, C.: Studio istofotometrico della metacromasia. a vari pH sulla medesima sezione istologica. Riv. Istoch. 10, 621–644 (1964).

    Google Scholar 

  • Collins, G. H., Cowden, R. R., Nevis, A. H.: Myoolonus epilepsy with Lafora bodies. Arch. Path. 86, 239–254 (1968).

    CAS  Google Scholar 

  • Curran, R. C.: The histochemistry of mucopolysaccharides. Int. Rev. Cytol. 17, 149–212 (1964).

    Article  CAS  Google Scholar 

  • Dubois-Dalcq, M.: Etude histochimique des corps de Lafora. Acta neuropath. (Berl.) 12, 205–217 (1969).

    Article  CAS  Google Scholar 

  • Fischer, E. R., Lillie, R. D.: The effect of methylation on basophilia. J. histochem. Cytochem. 2, 81–87 (1954).

    Article  Google Scholar 

  • Gabe, M.: Techniques histologiques. Paris: Masson 1968.

    Google Scholar 

  • Ganter, P., Jolles, G.: Histochimie normale et pathologique. Paris: Gauthier-Villars 1969.

    Google Scholar 

  • Geyer, G.: Der Sulfonsäurenachweis. Eine neue Methode für den histochemischen Nachweis von Sulfonsäuren. Acta histochem. (Jena) 14, 26–41 (1962).

    CAS  Google Scholar 

  • Glegg, R. E., Clermont, J., Leblond, C. P.: The use of lead tetraacetate, benzidine, o-di-anisidine and a “film test” in the investigating the periodic Schiff technique. Stain Technol. 27, 277–305 (1952).

    Article  CAS  Google Scholar 

  • Handbook of chemistry and physics R. C. Weast, Selbi, S. M., eds.). Cleveland: Chemical Rubber 1966–1967).

    Google Scholar 

  • Haust, D. M., Landing, B. H.: Analysis of reaction of phosphoryl chloride with tissue components. J. Histochem. Cytochem. 9, 548–552 (1961).

    Article  CAS  Google Scholar 

  • Heikop ten Ham, M. W.: Lafora disease: review of histochemical aspects. Arch. Neurobiol. (Madr.) 28, 647–666 (1965).

    Google Scholar 

  • Korhonen, L. K.: Specific methylation of carboxyl groups by thyonil chloride in methanol. Acta histochem. (Jena) 26, 80–86 (1967).

    CAS  Google Scholar 

  • Landing, B. H., Hall, H. E.: Phosphorylation as a histochemical procedure. J. Histochem. Cytochem. 4, 41–46 (1956).

    Article  CAS  Google Scholar 

  • Landsmeer, J. M. F.: Some colloid chemical aspects of methachromasia; influence of pH and salts on metachromatic phenomena evoked by toluidine blue in animal tissue. Acta pharmacol. neerl. 2, 112–128 (1951).

    CAS  Google Scholar 

  • Lillie, R. D.: Histopathologic technic and practical histochemistry. New York: McGraw-Hill 1965.

    Google Scholar 

  • Lison, L.: Histochemie et cytochemie animales. Paris: Gauthiers-Villars 1960.

    Google Scholar 

  • Luft, J. H.: Ruthenium red and violet. I∘: chemistry; purification methods of use for electron microscopy and mechanism of action. Anat. Rec. 171, 347–369 (1971a).

    Article  CAS  Google Scholar 

  • Luft, J. H.: II∘: fine structural localisation in animal tissue. Anat. Rec. 171, 369–417 (1971b).

    Article  CAS  Google Scholar 

  • Mangin: L.: Sur l'emploi du rouge de ruthénium en anatomie vegetale. C. R. Acad. Sci. (Paris) 66, 363–365 (1893).

    Google Scholar 

  • Marchetti, G.: Bario. In: Nuova Enc. Chimica (I. Guareschi, ed.), T. 4, p. 706–733. Torino: Utet 1924.

    Google Scholar 

  • Martin, J. J., Barcy, Th. de, Palladini, G.: La maladie de Pompe: Etude morphologique des lesions du système nerveux et des muscles striés (sous presse).

  • Mayer, P.: Über Schleinmfärbung. Mitt. zool. Stat. Neapel 12, 303–330 (1896).

    Google Scholar 

  • Mowry, R. W.: Improved procedure for the staining of acidic polysaccharides by Muller's colloidal (hydrous) ferric oxyde and its combination with the Feulgen and the periodic Schiff reactions. Lab. Invest. 7, 566–576 (1958a).

    CAS  Google Scholar 

  • Mowry, R. W.: Observations on the use of sulfuric acid in ether for the sulfatation of hydroxyl groups in tissue sections. J. Histochem. Cytochem. 6, 82–83 (1958b).

    CAS  Google Scholar 

  • Mowry, R. W.: The special value of methods that color both acidic and vicinal hydroxyl groups in the histochemical study of mucins. With revised directions for the colloidal iron stain, the use of Alcian blue 8GX and their combination with the periodic Schiff reaction. Ann. N.Y. Acad. Sci. 106, 402–423 (1963).

    Article  CAS  Google Scholar 

  • Palladini, G., Alfei, L.: Istochimica dei mucopolisaccaridi solforati: la reazione di Bracco-Curti. Riv. Istoch. 11, 51–60 (1965).

    Google Scholar 

  • Pearse, A. G. E.: Histochemistry, theoretical and applied. London: J. A. Churchill 1968.

    Google Scholar 

  • Rizzotti, M.: Sulla cromotropia di un polisaccaride fosforato presente nel secreto della ghiandola dell'albume di Helix terrestris. Riv. Istoch. 8, 287–293 (1962).

    Google Scholar 

  • Sakai, M., Austin, J., Witmer, F., Trueb, L.: Studies in myoclonus epilepsy (Lafora body form). II∘ Polyglucosans in the systemic deposits of myoclonus epilepsy and in corpora amylacea. Neurology (Minneap.) 20, 160–176 (1970).

    Article  CAS  Google Scholar 

  • Spicer, S. S.: A correlative study of the histochemioal properties of rodent acid mucopolysaocharides. J. Histochem. Cytochem. 8, 18–35 (1960).

    Article  CAS  Google Scholar 

  • Stempien, M. F.: Localisation of acid mucopolysaccharides with basic fuchsin. J. Histochem. Cytochem. 10, 766 (1962).

    Article  Google Scholar 

  • Stempien, M. F.: The use of a barium chloride-rhodizonate technique for the histochemical localisation of some sulphated acid mucopolysaccharides. J. Histochem. Cytochem. 11, 478–481 (1963).

    Article  CAS  Google Scholar 

  • Vilter, V.: Contribut à l'étude du mécanisme de la “méthylation saponification” dans l'histo-chimie des mucines acides. Ann. Histochem. 13, 205–220 (1968).

    CAS  Google Scholar 

  • Warren, L., Spicer, S. S.: Biochemical and histochemical identification of sialic acid containing mucins of rode vagina and salivary gland. J. Histochem. Cytochem. 9, 400–408 (1961).

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institut d'Anatomie Comparée “G. B. Grassi”, Université de Rome, Italy

    Guido Palladini

Authors
  1. Guido Palladini
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Palladini, G. Sur les réactions histochimiques des radicaux acides forts et notemment phosphate. Histochemie 30, 247–254 (1972). https://doi.org/10.1007/BF00277595

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00277595

Search

Navigation