Summary
TMV RNA contains a unique long segment lacking guanylic acid residues. Chromatographical, biochemical, and physical analyses suggest a size of about 40 nucleotides with one terminal 3′-Gp. Base composition of this stretch of TMV RNA appears to be strain-specific among three wild strains, yielding a general formula of (C8A20U11) G for the case of vulgare TMV, (C8A18U12) G for dahlemense TMV, and (C8A24U6) G for the strain U2. More closely related strains within the vulgare group (vulgare, A14, Ni 462) have no difference in base composition between these segments. Some new techniques are described which helped in determining the chain length of such long oligonucleotides. A decision whether this segment contains the beginning of the coat protein cistron on the TMV RNA or not had to be postponed until the nucleotide sequence is elaborated.
Similar content being viewed by others
References
Anderer, F. A., Wittmann-Liebold, B., Wittmann, H. G.: Weitere Untersuchungen zur Aminosäuren-Sequenz des Proteins des Tabakmosaikvirus. Z. Naturforsch. 20b, 1203–1213 (1965).
Funatsu, G., Tsugita, A., Fraenkel-Conrat, H.: Studies on the amino acid sequence of tobacco mosaic virus protein. V. Amino acid sequenences of two peptides from tryptic digests and location of amide group. Arch. Biochem. 105, 25–41 (1964).
Gooding, G. V., Hebert, T. T.: A simple technique for purification of tobacco mosaic virus in large quantities. Phytopathology 57, 1285 (1967).
Hiramaru, M., Uchida, T., Egami, F.: Ribonuclease preparation for the base analysis of polyribonucleotides. Analyt. Biochem. 17, 135–142 (1966).
Johnson, J.: Virus attenuation and the separation of strains by specific hosts. Phytopathology 37, 822–837 (1947).
Lindahl, T., Henley, D. D., Fresco, J.: Molecular weight and molecular weight distribution of unfractionated yeast transfer ribonucleic acid. J. Amer. chem. Soc. 87, 4961–4963 (1965).
Magasanik, B.: Isolation and composition of pentose nucleic acids and of corresponding nucleoproteins. In: The nucleic acids (E. Chargaff and N. J. Davidson, eds.), vol. I, p. 373–394. New York: Academic Press Inc. 1955.
Mandeles, St.: Location of unique sequences in tobacco mosaic virus ribonucleic acid. J. biol. Chem. 243, 3671–3674 (1968).
Melchers, G.: Die biologische Untersuchung des “Tomatenvirus Dahlem 1940”. Biol. Zbl. 60, 527–537 (1940).
Mundry, K. W.: The effect of nitrous acid on tobacco mosaic virus: mutation, not selection. Virology 9, 722–726 (1959).
—: A model of the coat protein cistron of tobacco mosaic virus and its biochemical investigation: the model, the experimental approach, and the isolation of a long oligonucleotide from TMV-RNA. Z. Vererbungsl. 97, 281–296 (1965).
—: Interactions between plant viruses and host cells. In: Reproduction: Molecular, subcellular, and cellular (M. Locke, ed.). New York: Academic Press Inc. 1965.
—: Automatic column-chromatographic microdetermination of nucleotides. In: Automation in analytical chemistry (L. Skeggs, ed.). New York: Mediad Inc. 1965.
—: Strain-specific sequences in TMV-RNA. Molec. Gen. Genetics 100, 383–384 (1967).
—, Gierer, A.: Die Erzeugung von Mutationen des Tabakmosaikvirus durch chemische Behandlung seiner Nucleinsäure in vitro. Z. Vererbungsl. 89, 614–630 (1958).
Schuster, H., Schramm, G.: Bestimmung der biologisch wirksamen Einheit des Tabakmosaikvirus auf chemischen Wege. Z. Naturforsch. 13b, 697–704 (1958).
Tomlinson, R. V., Tener, G. M.: The effect of urea, formamide, and glycols on the secondary binding forces in the ion exchange chromatography of polynucleotides on DEAE cellulose. Biochemistry 2, 692–702 (1963).
Wachter, R. de, Fiers, W.: Studies on the bacteriophage MS2. IV. The 3′-OH terminal undecanucleotide sequence of the viral RNA chain. J. molec. Biol. 30, 507–527 (1967).
Wittmann, H. G.: Proteinuntersuchungen an Mutanten des Tabakmosaikvirus als Beitrag zum Problem des genetischen Codes. Z. Vererbungsl. 93, 491–530 (1962).
—: Die primäre Proteinstruktur von Stämmen des Tabakmosaikvirus. Teil IV: Aminosäuresequenzen (1–61 und 135–158) des Proteins des Tabakmosaikvirus-Stammes U2. Z. Naturforsch. 20b, 1213–1223 (1965).
Author information
Authors and Affiliations
Additional information
Communicated by H. G. Wittmann
Rights and permissions
About this article
Cite this article
Mundry, K.W. Structural elements of viral ribonucleic acid and their variation. Molec. Gen. Genet. 105, 361–377 (1969). https://doi.org/10.1007/BF00277590
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00277590