Summary
The quantitative description of the morphology of chromatin of Feulgen-stained lymphocytes were done by means of the Image Analysing Computer Quantimet. The description consisted of the measurements of the areas of chromatin with different optical density. Three types of cells were analysed: resting lymphocytes, lymphocytes stimulated in vitro with phytohaemagglutinin, and lymphocytes stimulated in vitro with Concanavalin A. The differences in the area representation of the optical density of chromatin between three types of lymphocytes were found. The application of the method for the study of the relationship between the morphology of chromatin and the functional state of the cell is suggested.
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References
Brown, S. W.: Heterochromatin. Science 151, 417–425 (1966).
Chayen, J., Bitensky, L., Butcher, R., Poulter, L.: A guide to practical histochemistry, p. 57. Edinburgh: Oliver and Boyd 1969.
Decosse, J. J., Aiello, N.: Feulgen hydrolysis: effect of acid and temperature. J. Histochem. Cytochem. 14, 601–604 (1966).
Fisher, C., Cole, C.: The metals research image analysing computer. Microscope 16, 81–94 (1968).
Itikawa, O., Ogura, Y.: The Feulgen reaction after hydrolysis at room temperature. Stain Technol. 29, 13–15 (1954).
Kiliovska, M., Radéva, V.: Sur la non -homogeneite optique de noyau cellulaire dans le processus de differenciation du tissu nerveux. C. R. Acad. bulg. Sci. 22, 831–834 (1969).
Kiliovska, M., Radéva, V.: Recherches cytophotometriques sur la non-homogeneite optique du noyau cellulaire dans le processus de differenciation du chorda dorsalis du Triturus vulgaris. C. R. Acad. bulg. Sci. 24, 393–395 (1971).
Konwiński, M., Kozlowski, T.: Morphometric study of normal and phytohemagglutinin-stimulated lymphocytes. Z. Zellforsch. 129, 500–507 (1972)
Ling, N.R.: Lymphocyte stimulation. Amsterdam: North-Holland Publishing Company 1968.
Mertz, M., Hinrichsen, K.: Erfassung morphologischer Merkmale von Zellkernen mittels bildanalytischer Meßdaten. I. Versuch zur densitometrischen Eichbarkeit des Quantimet. Prakt. Metallographie 6, 676–688 (1969a).
Mertz, M., Hinrichsen, K.: Erfassung morphologischer Merkmale von Zellkernen mittels bildanalytischer Meßdaten. II. Die Flächenrepräsentanz der Absorptionen. Prakt. Metallographie 6, 711–722 (1969b).
Powell, A. E., Leon, M. A.: Reversible interaction of human lymphocytes with the mitogen concanavalin A. Exp. Cell Res. 62, 315–325 (1970).
Sandritter, W., Kiefer, G., Schlüter, G., Moore, W.: Eine cytophotometrische Methode zur Objektivierung der Morphologie von Zellkernen. Ein Beitrag zum Problem von Eu- und Heterochromatin. Histochemie 10, 341–352 (1967).
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Rowiński, J., Pieńkowski, M. & Abramczuk, J. Area representation of optical density of chromatin in resting and stimulated lymphocytes as measured by means of quantimet. Histochemie 32, 75–80 (1972). https://doi.org/10.1007/BF00277473
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DOI: https://doi.org/10.1007/BF00277473