Summary
Mast cell granules were shown to contain an apparently branched meshwork of fibers, which had a diameter of about 240 Å and a denser core of about 20–30 Å. Mast cell granules from rats were found to have a median weight of 39×10−14 g after critical-point drying. Their dry mass increased 23% when stained with ruthenium red at pH 5.0. The staining reaction was found to be stoichiometric, and the bulk of the stain appeared to be taken up by heparin in a molar relationship of one ruthenium red cationic complex per sulfate group of heparin. The uptake of the stain was largely localized to the cores of the granule fibers, which after staining appeared double contoured. These findings suggest that mast cell heparin is integrated into the fibrous structure of the mast cell granules.
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This project was supported in part by Grant No. P-259-H from the American Cancer Society.
The opinions or assertions contained herein are the private views of the author and are not to be construed as official or as reflecting the views of the Department of the Army or the Department of Defense.
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Pihl, E. A quantitative electron histochemical estimation of ruthenium red binding to heparin in mast cell granules. Histochemie 22, 302–315 (1970). https://doi.org/10.1007/BF00277458
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DOI: https://doi.org/10.1007/BF00277458