Summary
Peroxidase activity has been localized to duct cells of the submandibular salivary gland of the hamster using a 3,3′-diaminobenzidine (DAB)-H2O2 medium. In cryostat sections of glutaraldehyde-fixed tissue the enzyme activity is found in the proximal part of the duct system of the gland. In Epon sections studied in the light microscope or thin sections studied in the electron microscope the peroxidase activity is observed in cytoplasmic granules in cells of the convoluted tubules of the ducts. No activity is seen in the acini or in cells of the intralobular striated ducts. The submandibular gland of the rat was negative with respect to peroxidase reaction. The findings are discussed with special reference to the possible correlation between peroxidase activity and iodine metabolism in salivary glands.
Similar content being viewed by others
References
Bainton, D.F., Farquhar, M.G.: Differences in enzyme content of azurophil-and specific granules of polymorphqnuclear leukocytes. J. Cell Biol. 39, 299–317 (1968).
Bancroft, G., Elliott, K.A.C.: Tne distribution of peroxidase in animal tissues. Biochem. J. 28, 1911–1919 (1934).
Beard, M.E., Kovikoff, A.B.: Distribution of perxoisomes (microbodies) in the nephron of the rat. J. Cell Biol. 42, 501–518 (1969).
Brown-Grant, K.: Extrathyroidal iodide concentrating mechanisms. Physiol. Rev. 41, 189–213 (1961).
Burstone, M.S.: Enzyme histochemistry and its application in the study of neoplasms. New York and London: Acad. Press 1962.
Cohen, B., Myant, N.B.: Concentration of salivary iodide. A comparative study. J. Physiol. (Lond.) 145, 595–610 (1959).
—, Logothetopoulos, J.H.: Autoradiografic localization of iodine-131 in the salivary glands of the hamster. Nature (Lond.) 176, 1268–1269 (1955).
DeDuve, C., Baudhuin, P.: Peroxisomes (microbodies and related particles). Physiol. Rev. 46, 323–357 (1966).
Devi, N.S., Jacoby, F.: The submaxillary gland of the golden hamster and its post-natal development. J. Anat. (Lond.) 100, 269–285 (1966).
Fahimi, H.D.: Cytochemical localization of peroxidase activity in rat hepatic microbodies (peroxisomes). J. Histochem. Cytochem. 16, 547–550 (1968).
Hati, R.N., Bal, M., Datta, A.G.: A potassium iodide stimulated peroxidase from goat submaxillary gland. Biochem. biophys. Res. Commun. 31, 392–397 (1968).
—, Datta, A.G.: Studies on the formation of diiodotyrosine from monoidotyrosine by a microsomal enzyme from goat submaxillary gland. Biochim. biophys. Acta (Amst.) 148, 310–312 (1967).
Klebanoff, S.J.: Myeloperoxidase-halide-hydrogen peroxide antibacterial system. J. Bact. 95, 2131–2138 (1968).
Kumlien, A.: Iodine metabolism and peroxidase activity in salivary glands. (To be published).
- An improved method for the autoradiography of 125I-in tissue sections. (To be published).
Liberman, E.A., Topaly, V.P., Tsofina, L.M., Shkrob, A.M.: Transport of iodide through artificial phospholipid membranes. Biofizika 14, 56–61 (1969).
Ljunggren, J.-G.: The formation of thyroxine from diiodothyrosine in the presence of horseradish-peroxidase and hydrogen peroxide. Biochim. biophys. Acta (Amst.) 107, 434–440 (1965).
—: The catalytic effect of peroxidases on the iodination of thyrosine in the presence of hydrogen peroxide. Biochim. biophys. Acta (Amst.) 113, 71–78 (1966).
—, Åkesson, Å.: Solubilization, isolation and identification of a peroxidase from the microsomal fraction of beef thyroid. Arch. Biochem. 127, 346–353 (1968).
Logothetopoulos, J.H., Myant, N.B.: Concentration of radio-iodide and 35S-thiocyanate by the salivary glands. J. Physiol. (Lond.) 134, 189–194 (1956).
Luft, J.H.: Improvements in Epon resin embedding methods. J. biophys. biochem. Cytol. 9, 409–414 (1961).
Margoliash, E., Novogrodsky, A.: Inhibition of catalase by aminotriazole. Biochem. J. 68, 468–475 (1958).
MoGee, J.O'D., Mason, D.K., Duguid, W.P.: The site of iodide concentration in hamster salivary glands as demonstrated by autoradiography. Arch. oral Biol. 12, 1189–1193 (1967).
Morrison, M., Allen, P.Z.: The identification and isolation of lactoperoxidase from salivary glands. Biochem. biophys. Res. Commun. 13, 490–494 (1963).
- Rombauts, W.A., Schroeder, W.A.: Lactoperoxidase. In: Hemes and Hemoproteins, ed. B. Chance. 345–348. Acad. Press 1966.
Novikoff, A.B., Goldfischer, S.: Visualization of peroxisomes (microbodies) and mitochondria with diaminobenzidine. J. Histochem. Cytochem. 17, 675–680 (1969).
Saunders, B.C., Stark, B.P.: Studies in peroxidase action. XII. Transiodination and related processes. Tetrahedron 4, 169–177 (1958).
Shapiro, B.L.: A morphological and histochemical study of three oxidative enzymes in the hamster submandibular gland. Arch. oral Biol. 12, 1053–1061 (1967).
Taurog, A., Potter, G.P., Chaikoff, I.L.: The effect of hypophysectomy and of TSH on the mouse submaxillary iodide pump. Endocrinology 64, 1038–1051 (1959).
Thomson, J., Morell, D.B.: The structure, location and distribution of salivary gland peroxidase. J. Biochem. (Tokyo) 62, 483–486 (1967).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Bloom, G.D., Carlsöö, B. & Kumlien, A. Cytochemical localization of peroxidase activity in the submandibular salivary gland of the hamster. Histochemie 22, 294–301 (1970). https://doi.org/10.1007/BF00277457
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00277457