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Isolation of a transducing phage carrying rpsT, the structural gene for ribosomal protein S20

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Summary

λ transducing phages carrying segments of the Escherichia coli chromosome in the dapB region have been isolated and their in vivo gene products analyzed by two-dimensional gel electrophoresis. One of these phages, λddapB-2, carries the structural genes for ribosomal protein S20 (rpsT) and isoleucyltransfer RNA synthetase (ileS). The most likely gene order is thr-rpsT-ileS-dapB-pyrA.

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Authors and Affiliations

  1. Department of Biology, York University, 4700 Keele Street, M3J 1P3, Downsview, Ontario, Canada

    James D. Friesen, Jack Parker & Robert J. Watson

  2. University Institute of Microbiology, Øster Farimagsgade 2A, DK-1353, Copenhagen K, Denmark

    Niels P. Fiil

  3. Institute of Molecular Biology, University of Aarhus, Aarhus, Denmark

    Steen Pedersen

Authors
  1. James D. Friesen
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  2. Jack Parker
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  3. Robert J. Watson
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  4. Niels P. Fiil
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  5. Steen Pedersen
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Communicated by H.G. Wittmann

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Friesen, J.D., Parker, J., Watson, R.J. et al. Isolation of a transducing phage carrying rpsT, the structural gene for ribosomal protein S20. Molec. Gen. Genet. 144, 115–118 (1976). https://doi.org/10.1007/BF00277314

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  • DOI: https://doi.org/10.1007/BF00277314

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