Summary
In crosses of [ANT R8] with auxotrophic strains, resistance to antimycin segregates almost 50:50 in random spore analysis with a slight preponderance for the sensitivity allele. Tetrad analysis, however, shows all possible types of tetrads (2:2; 3:1; 1:3; 4:0; 0:4 resistant versus sensitive) with an excess of 2:2 segregations and sectoring of colonies on antimycin medium indicating an extrachromosomal mode of inheritance. The overall ratio of resistant versus sensitive spores is the same as compared with random spore data.
Using a mutant blocked in meiosis (mei 1) mitotic segregation of stable diploids is achieved, leading to a ratio of 20% resistant to 80% sensitive clones. Possible reasons for the bias in transmission of the resistance determinant are discussed.
Mitotic haploidization experiments reveal no linkage of the [ANT R8] mutational site to markers on any of the three on to now known chromosomes and disagrees so far with the possibility of a nuclear mutation necessary for expression of the extrachromosomal mutation.
Growing the mutant [ANT R8] and/or the wildtype in the presence of ethidium bromide (EB) before mating, one can change the ratio of resistant versus sensitive diploids, indicating a multiple number of resistance determinants.
The sum of all results makes it very likely that [ANT R8] is a mitochondrial mutation.
In addition, the paper presents the methodology for extrachromosomal genetics in Schizosaccharomyces pombe.
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Wolf, K., Burger, G., Lang, B. et al. Extrachromosomal inheritance in Schizosaccharomyces pombe . Molec. Gen. Genet. 144, 67–73 (1976). https://doi.org/10.1007/BF00277307
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DOI: https://doi.org/10.1007/BF00277307