Summary
The effect on translational fidelity of a particular mutation in the gene coding for protein S5 (rpxE) has been investigated. This mutation has the opposite effect of a restrictive strA mutation; in vivo, it relieves the restriction imposed by strA on the suppression of T4 nonsense mutants and results in hypersensitivity to streptomycin; in vitro, the presence of the altered S5 protein in 30S ribosomes results in increased intrinsic misreading. It is concluded that this mutation, ramC319, acts as a ribosomal ambiguity mutation similar to certain mutations of protein S4 (ramA).
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Communicated by H.G. Wittmann
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Cabezón, T., Herzog, A., De Wilde, M. et al. Cooperative control of translational fidelity by ribosomal proteins in Escherichia coli . Molec. Gen. Genet. 144, 59–62 (1976). https://doi.org/10.1007/BF00277305
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DOI: https://doi.org/10.1007/BF00277305