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Amino acid replacement in the protein S5 from a spectinomycin resistant mutant of Bacillus subtilis

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Summary

Ribosomal protein S5 was isolated from wild type Bacillus subtilis ATCC 6633 and from a spectinomycin resistant mutant (BSPC 111) derived from spectinomycin sensitive to resistance is accomtrypsin and all the tryptic peptides were isolated by column- and paper-chromatography. By comparative amino acid analyses of the peptides, it was demonstrated that the S5 from the mutant differs from the wild type S5 by a replacement of one amino acid, namely lysine by isoleucine in the peptide T9. The results are compared with E. coli spectinomycin resistant mutants.

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Authors and Affiliations

  1. Department of Biochemistry and Biophysics, Research Institute for Nuclear Medicine and Biology, Hiroshima University, Kasumi-Cho, 734, Hiroshima, Japan

    T. Itoh

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  1. T. Itoh
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Communicated by H.G. Wittmann

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Itoh, T. Amino acid replacement in the protein S5 from a spectinomycin resistant mutant of Bacillus subtilis . Molec. Gen. Genet. 144, 39–42 (1976). https://doi.org/10.1007/BF00277301

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  • DOI: https://doi.org/10.1007/BF00277301

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