Summary
Genetic analysis showed that the glycerol non-utilizing isolate gly-u(234) of Neurospora crassa is derived by mutation in a nuclear gene situated in the right arm of linkage group I, about 2.2 crossover units distal to ad-9 and 11 units proximal to nit-1.
Enzymatic testings using a radiochemical method indicate that the mutant is deficient for the enzyme glycerol kinase. The radiochemical testings further indicate that the mutation has inactivated an inducible glycerol kinase, while a low residual activity may be due to a second, basal and non-inducible glycerol kinase, in accordance with a proposal by North (1973, 1974) that Neurospora has two glycerol kinases with these properties.
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Communicated by F. Kaudewitz
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Holm, K., Nilheden, E., Kølmark, H.G. et al. Genetic and enzymatic analysis of a glycerol kinase deficient mutant in Neurospora crassa . Molec. Gen. Genet. 144, 11–15 (1976). https://doi.org/10.1007/BF00277297
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DOI: https://doi.org/10.1007/BF00277297