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Micro-scale two-dimensional polyacrylamide gel electrophoresis of ribosomal proteins

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Summary

A specially designed apparatus and conditions are described for the rapid analysis of ribosomal proteins by two-dimensional gel electrophoresis on a micro scale. The resolution of proteins in electropherograms is comparable to that obtained with other systems, but because of miniaturization, only 0.5 to 1 μg of each protein is required, and the entire procedure, including electrophoresis in both dimensions, and staining and destaining can be completed in 6 to 7 hours.

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Authors and Affiliations

  1. Department of Biochemistry, University of Chicago, 920 East 58th Street, 60637, Chicago, Illinois, USA

    Alan Lin, Ekkehard Collatz & Ira G. Wool

Authors
  1. Alan Lin
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  2. Ekkehard Collatz
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  3. Ira G. Wool
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Communicated by H.G. Wittmann

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Lin, A., Collatz, E. & Wool, I.G. Micro-scale two-dimensional polyacrylamide gel electrophoresis of ribosomal proteins. Molec. Gen. Genet. 144, 1–9 (1976). https://doi.org/10.1007/BF00277296

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  • DOI: https://doi.org/10.1007/BF00277296

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