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Application of silver stains to gastric endocrine cells in plastic sections

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Summary

A simultaneous light and electron microscopic study of mouse gastric mucosa was made to determine whether the silver nitrate methenamine stain of Duk-Ho Lee could be used to stain gastric endocrine-like cells in plastic embedded tissue. Examination of consecutive thick and thin sections showed that this stain blackened the granules of the predominant type of endocrine-like cell present. Blackening of the granules with silver occured in tissue fixed in osmium tetroxide solution with or without dichromate salt or in tissue fixed in glutaraldehyde then treated with osmium. The intensity of staining was deepest in the osmium-dichromate fixed tissue, but the glutaraldehyde-osmium procedure gave less interference from diffuse silver impregnation and better preservation of detail for electron microscopy.

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References

  • Bodian, D.: A new method for staining nerve fibers and nerve endings in mounted paraffin sections. Anat. Rec. 65, 89–97 (1936).

    Google Scholar 

  • Busolati, C., Pearse, A. G. E.: Immunofluorescent localization of the gastrin secreting G cell in the pyloric antrum of the pig. Histochemie 21, 1–4 (1970).

    Google Scholar 

  • Forssman, W. G., Orci, L., Pictet, R., Renold, A. E., Rouiller, C.: The endocrine cells in the epithelium of the gastrointestinal mucosa of the rat. J. Cell Biol. 40, 692–715 (1969).

    Google Scholar 

  • Lee, Duk-Ho: Identification of argyrophilic cells in pancreatic islets by light and electron microscopy in osmium fixed plastic-embedded sections. Z. Zellforsch. 77, 1–7 (1967).

    Google Scholar 

  • Pearse, A. G. E., Coulling, I., Weavers, B., Friesen, S.: The endocrine polypeptide cells of the human stomach, duodenum, and jejunum. Gut 2, 649–658 (1970).

    Google Scholar 

  • Pease, D.: Histological techniques for electron microscopy, 2nd ed. New York-London: Academic Press 1964.

    Google Scholar 

  • Vassallo, G., Solcia, E., Capella, C.: Light and electron microscopic identification of several types of endocrine cells in the gastrointestinal mucosa of the cat. Z. Zellforsch. 98, 333–356 (1969).

    Google Scholar 

  • Weinshelbaum, E. I., Ferguson, D. J.: Localization of tritium from histamine in gastric mucosa in vivo. Histochemie 26, 9–19 (1971).

    Google Scholar 

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Authors and Affiliations

  1. Department of Surgery, Veterans Administration Hospital, Lake City, Florida

    Edward I. Weinshelbaum & Jan M. Pittman

  2. The Veterans Administration Hospital, Gainesville, Florida

    Edward I. Weinshelbaum & Jan M. Pittman

Authors
  1. Edward I. Weinshelbaum
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  2. Jan M. Pittman
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Weinshelbaum, E.I., Pittman, J.M. Application of silver stains to gastric endocrine cells in plastic sections. Histochemie 29, 134–139 (1972). https://doi.org/10.1007/BF00277281

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  • DOI: https://doi.org/10.1007/BF00277281

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